Detailed information of TA system TA031810

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2170862..2171087
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ECpv15279_RS11215
• Protein ID: WP_000813258.1
• Coordinates: 2170862..2171017 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2171029..2171087 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS11180	2166747..2167460	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ECpv15279_RS11185	2167596..2167793	-	198	WP_000917763.1	hypothetical protein	-
ECpv15279_RS11190	2168018..2168572	-	555	WP_000640035.1	DUF1133 family protein	-
ECpv15279_RS11195	2168581..2168940	-	360	WP_001217447.1	RusA family crossover junction endodeoxyribonuclease	-
ECpv15279_RS11200	2168953..2170002	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ECpv15279_RS11205	2170004..2170276	-	273	WP_000191872.1	hypothetical protein	-
ECpv15279_RS11210	2170398..2170742	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ECpv15279_RS11215	2170862..2171017	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2171029..2171087	+	59	-	-	Antitoxin
ECpv15279_RS11220	2171308..2171865	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ECpv15279_RS11225	2171867..2172085	-	219	WP_000683609.1	DUF4014 family protein	-
ECpv15279_RS11230	2172213..2172524	-	312	WP_001289672.1	hypothetical protein	-
ECpv15279_RS11235	2172517..2172732	-	216	WP_050554342.1	hypothetical protein	-
ECpv15279_RS11240	2172721..2173934	+	1214	WP_162829202.1	IS3 family transposase	-
ECpv15279_RS11245	2173976..2174509	+	534	Protein_2127	tyrosine-type recombinase/integrase	-
ECpv15279_RS11255	2174697..2175716	-	1020	WP_000836042.1	Zn-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa / nleC / nleC / stx2B / stx2A / espM1 / nleH2 / nleA/espI	1989300..2174509	185209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T31810 WP_000813258.1 NZ_AP018488:c2171017-2170862 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T31810 NZ_AP018488:c2171017-2170862 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT31810 NZ_AP018488:2171029-2171087 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin