Detailed information of TA system TA031805

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1661516..1661741
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECpv15279_RS08315
• Protein ID: WP_000813254.1
• Coordinates: 1661586..1661741 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1661516..1661574 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS08265	1656667..1657074	-	408	WP_001003379.1	helix-turn-helix domain-containing protein	-
ECpv15279_RS08270	1657152..1657379	+	228	WP_000476986.1	transcriptional regulator	-
ECpv15279_RS08275	1657363..1657914	+	552	WP_000705370.1	hypothetical protein	-
ECpv15279_RS08280	1657886..1658926	+	1041	WP_000020565.1	DNA-binding protein	-
ECpv15279_RS08285	1658838..1659380	+	543	WP_157837342.1	replication protein	-
ECpv15279_RS08290	1659415..1660173	+	759	WP_000537579.1	DUF1627 domain-containing protein	-
ECpv15279_RS08295	1660233..1660418	+	186	WP_000215514.1	hypothetical protein	-
ECpv15279_RS08310	1660766..1661314	+	549	WP_000211435.1	ORF6N domain-containing protein	-
-	1661516..1661574	-	59	-	-	Antitoxin
ECpv15279_RS08315	1661586..1661741	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ECpv15279_RS08320	1661844..1662161	-	318	WP_000042395.1	DNA-binding transcriptional regulator	-
ECpv15279_RS08325	1662154..1662525	-	372	WP_001217944.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ECpv15279_RS08330	1662749..1662976	+	228	WP_001452497.1	hypothetical protein	-
ECpv15279_RS08335	1663030..1663299	+	270	WP_024177817.1	hypothetical protein	-
ECpv15279_RS08340	1663301..1664350	+	1050	WP_001265189.1	DUF968 domain-containing protein	-
ECpv15279_RS08345	1664363..1664737	+	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
ECpv15279_RS08350	1664734..1665555	+	822	WP_000762902.1	antitermination protein	-
ECpv15279_RS08355	1665782..1665979	+	198	WP_000917731.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	1649263..1695828	46565

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T31805 WP_000813254.1 NZ_AP018488:1661586-1661741 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T31805 NZ_AP018488:1661586-1661741 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATTGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT31805 NZ_AP018488:c1661574-1661516 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin