Detailed information of TA system TA031800

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1163580..1163805
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ECpv15279_RS05520
• Protein ID: WP_000813263.1
• Coordinates: 1163650..1163805 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1163580..1163638 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS05480	1159057..1159293	+	237	Protein_1047	DUF977 family protein	-
ECpv15279_RS05485	1159332..1160870	-	1539	WP_000998048.1	IS66-like element ISEc8 family transposase	-
ECpv15279_RS05490	1160920..1161267	-	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
ECpv15279_RS05495	1161264..1161644	-	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
ECpv15279_RS05500	1161738..1161920	+	183	Protein_1051	DUF977 family protein	-
ECpv15279_RS05505	1162272..1163045	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1163580..1163638	-	59	-	-	Antitoxin
ECpv15279_RS05520	1163650..1163805	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
ECpv15279_RS05525	1163973..1164251	+	279	WP_001341388.1	hypothetical protein	-
ECpv15279_RS05530	1164253..1165302	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
ECpv15279_RS05535	1165315..1165686	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ECpv15279_RS05540	1165676..1166047	+	372	WP_000090264.1	antitermination protein	-
ECpv15279_RS05545	1166199..1167017	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ECpv15279_RS05550	1167304..1167500	+	197	Protein_1059	TrmB family transcriptional regulator	-
ECpv15279_RS05555	1167638..1168351	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T31800 WP_000813263.1 NZ_AP018488:1163650-1163805 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T31800 NZ_AP018488:1163650-1163805 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT31800 NZ_AP018488:c1163638-1163580 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin