Detailed information of TA system TA031796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 293637..294331
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ECpv15279_RS01335
• Protein ID: WP_001263495.1
• Coordinates: 293933..294331 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ECpv15279_RS01330
• Protein ID: WP_000554757.1
• Coordinates: 293637..293930 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS01310	289348..289845	+	498	Protein_254	REP-associated tyrosine transposase RayT	-
ECpv15279_RS01315	289990..291702	-	1713	Protein_255	flagellar biosynthesis protein FlhA	-
ECpv15279_RS01320	291674..292459	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ECpv15279_RS01325	292530..293585	+	1056	WP_001226186.1	DNA polymerase IV	-
ECpv15279_RS01330	293637..293930	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ECpv15279_RS01335	293933..294331	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ECpv15279_RS01340	294341..294793	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ECpv15279_RS01345	295112..295315	+	204	Protein_261	RNA ligase RtcB family protein	-
ECpv15279_RS01350	295314..295835	+	522	Protein_262	peptide chain release factor H	-
ECpv15279_RS01355	295892..297349	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ECpv15279_RS01360	297610..298068	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	298664..298744	+	81	NuclAT_11	-	-
-	298664..298744	+	81	NuclAT_11	-	-
-	298664..298744	+	81	NuclAT_11	-	-
-	298664..298744	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T31796 WP_001263495.1 NZ_AP018488:293933-294331 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T31796 NZ_AP018488:293933-294331 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT31796 WP_000554757.1 NZ_AP018488:293637-293930 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT31796 NZ_AP018488:293637-293930 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6