Detailed information of TA system TA031779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 44096..44612
• Replicon: plasmid pLPS-2
• Accession: NZ_AP018394
• Organism: Lacticaseibacillus paracasei strain IJH-SONE68

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: EL355_RS16460
• Protein ID: WP_126353757.1
• Coordinates: 44096..44377 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: EL355_RS16465
• Protein ID: WP_016369696.1
• Coordinates: 44370..44612 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL355_RS16435	40440..41297	+	858	WP_003569650.1	sugar ABC transporter permease	-
EL355_RS16440	41481..42437	+	957	WP_003569652.1	LacI family transcriptional regulator	-
EL355_RS16445	42496..42627	-	132	Protein_47	helix-turn-helix domain-containing protein	-
EL355_RS16450	43019..43303	-	285	WP_016383626.1	BRCT domain-containing protein	-
EL355_RS16455	43309..43959	-	651	WP_003586220.1	recombinase family protein	-
EL355_RS16460	44096..44377	-	282	WP_126353757.1	type II toxin-antitoxin system YafQ family toxin	Toxin
EL355_RS16465	44370..44612	-	243	WP_016369696.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EL355_RS16470	44918..45197	+	280	Protein_52	HTH domain-containing protein	-
EL355_RS16485	45582..46471	+	890	Protein_53	IS30 family transposase	-
EL355_RS16490	46524..47684	+	1161	Protein_54	IS3 family transposase	-
EL355_RS16500	47762..48102	+	341	Protein_55	transposase	-
EL355_RS16505	48140..48916	+	777	Protein_56	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51020	51020
	-	flank	IS/Tn	-	-	47064..47684	620

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (9-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-63)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11348.90 Da
• Isoelectric Point: 9.1886

>T31779 WP_126353757.1 NZ_AP018394:c44377-44096 [Lacticaseibacillus paracasei]
MNNFIYKPAFERQFKKNYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T31779 NZ_AP018394:c44377-44096 [Lacticaseibacillus paracasei]
ATGAATAACTTTATTTACAAACCAGCCTTTGAACGTCAATTTAAAAAAAATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGACTTTGAAGCTGTTTATCATAAACTATTAAGAGATGAACCGTTAGATCCACATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9244.47 Da
• Isoelectric Point: 5.1548

>AT31779 WP_016369696.1 NZ_AP018394:c44612-44370 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLNRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT31779 NZ_AP018394:c44612-44370 [Lacticaseibacillus paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAATCGTGTGGGATTAAC
TCCCGGAGAAGCCTTTCGTATCTTTGCCAAGAAATCCATTGAGGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGACTGGATTGGTTAAATGAA
TAA