Detailed information of TA system TA031777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 2931..3447
• Replicon: plasmid pLPS-1
• Accession: NZ_AP018393
• Organism: Lacticaseibacillus paracasei strain IJH-SONE68

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: EL355_RS15950
• Protein ID: WP_003573774.1
• Coordinates: 2931..3212 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: EL355_RS15955
• Protein ID: WP_016363783.1
• Coordinates: 3205..3447 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL355_RS15925	358..1251	+	894	WP_126353716.1	DNA/RNA non-specific endonuclease	-
EL355_RS15930	1256..1615	+	360	WP_079351839.1	transcriptional regulator	-
EL355_RS15935	1635..1946	+	312	WP_126353718.1	glutaredoxin family protein	-
EL355_RS15945	2343..2762	-	420	WP_126353720.1	hypothetical protein	-
EL355_RS15950	2931..3212	-	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
EL355_RS15955	3205..3447	-	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EL355_RS15960	3717..4286	-	570	Protein_6	ISL3 family transposase	-
EL355_RS15965	4309..5229	-	921	WP_126353722.1	IS30 family transposase	-
EL355_RS15970	5394..6098	-	705	WP_103220218.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45267	45267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T31777 WP_003573774.1 NZ_AP018393:c3212-2931 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T31777 NZ_AP018393:c3212-2931 [Lacticaseibacillus paracasei]
ATGAATAAATTTGTTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACGAGATGAACCGTTAGATCCGCATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT31777 WP_016363783.1 NZ_AP018393:c3447-3205 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT31777 NZ_AP018393:c3447-3205 [Lacticaseibacillus paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGGTTAAC
TCCCGGGGAAGCTTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGACTGGATTGGTTAAATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7