Detailed information of TA system TA031745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 468213..468861
• Replicon: chromosome
• Accession: NZ_AP018338
• Organism: Streptococcus oralis subsp. tigurinus strain osk_001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: STO1_RS02325
• Protein ID: WP_096421794.1
• Coordinates: 468213..468578 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: E0PPW3
• Locus tag: STO1_RS02330
• Protein ID: WP_000797751.1
• Coordinates: 468568..468861 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STO1_RS02310	464127..467171	+	3045	WP_096422976.1	type I restriction endonuclease subunit R	-
STO1_RS02315	467304..467708	+	405	WP_096421790.1	YbgA family protein	-
STO1_RS02320	467692..468057	+	366	WP_096421792.1	TIGR02328 family protein	-
STO1_RS02325	468213..468578	+	366	WP_096421794.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
STO1_RS02330	468568..468861	+	294	WP_000797751.1	helix-turn-helix transcriptional regulator	Antitoxin
STO1_RS02335	469122..471371	+	2250	WP_096421796.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
STO1_RS02340	471466..472332	+	867	WP_002877494.1	methylenetetrahydrofolate reductase [NAD(P)H]	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14608.83 Da
• Isoelectric Point: 10.0748

>T31745 WP_096421794.1 NZ_AP018338:468213-468578 [Streptococcus oralis subsp. tigurinus]
VHTIYFYKDKNGNEPVLDYMRELARKKSKDSRIKLNKLNDYIELLSQHGTRAGEPYIKHLEDEIWELRPLRDRILFVAWL
DGSFVFLHHFVKKTQKTPRREIEKAKRELKDLKERGLSDEK

Nucleotide

• Length: 366 bp

>T31745 NZ_AP018338:468213-468578 [Streptococcus oralis subsp. tigurinus]
GTGCATACAATTTACTTCTATAAGGACAAAAATGGAAATGAGCCAGTCTTAGATTATATGAGAGAATTGGCTAGAAAGAA
GAGTAAGGATAGTCGCATTAAACTGAACAAACTAAACGACTATATTGAGTTGCTTAGTCAGCATGGGACTAGAGCTGGTG
AACCCTATATTAAACATTTGGAAGATGAAATTTGGGAACTAAGACCTCTGAGGGATAGGATTTTATTTGTAGCATGGCTT
GATGGAAGTTTTGTTTTCTTACATCATTTTGTCAAAAAGACTCAGAAAACTCCTAGGAGAGAAATTGAAAAAGCCAAGCG
TGAACTGAAGGACTTAAAAGAAAGAGGATTAAGTGATGAAAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10752.41 Da
• Isoelectric Point: 5.1991

>AT31745 WP_000797751.1 NZ_AP018338:468568-468861 [Streptococcus oralis subsp. tigurinus]
MKNSAIGSNWKDVRTELFTKEEILESDMRVAIMSELIEARHEQGISQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQEKS

Nucleotide

• Length: 294 bp

>AT31745 NZ_AP018338:468568-468861 [Streptococcus oralis subsp. tigurinus]
ATGAAAAATAGCGCCATTGGGAGTAATTGGAAGGATGTCCGAACAGAACTCTTTACCAAGGAAGAAATTCTTGAAAGTGA
TATGCGAGTGGCTATCATGAGTGAGTTGATAGAAGCTAGACATGAGCAAGGTATTAGTCAGAAAAAGCTAGAAGAACTCA
GTGGAGTAAGCCAGCCTGTTATAGCTAGGATGGAGACAGGAAAGACCAGTCCTCAGTTGGATACGGTCTTAAAAGTCTTA
GCCAGTTTAGGAAAGACACTAGCAGTCGTCCCACTTGAACAGGAAAAAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A224A4A2