Detailed information of TA system TA031693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2674069..2674661
• Replicon: chromosome
• Accession: NZ_AP018052
• Organism: Thiohalobacter thiocyanaticus strain FOKN1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CFK21_RS12335
• Protein ID: WP_096366942.1
• Coordinates: 2674380..2674661 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CFK21_RS12330
• Protein ID: WP_096367609.1
• Coordinates: 2674069..2674368 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFK21_RS12295	2669242..2670153	+	912	WP_096366935.1	phosphorylase	-
CFK21_RS12300	2670168..2670410	-	243	WP_096366936.1	DUF4282 domain-containing protein	-
CFK21_RS12305	2670465..2671100	-	636	WP_096366937.1	LysE family translocator	-
CFK21_RS12310	2671213..2671629	-	417	WP_096366938.1	hypothetical protein	-
CFK21_RS15425	2671956..2672216	-	261	WP_197702955.1	PhzF family phenazine biosynthesis protein	-
CFK21_RS12315	2672451..2672990	-	540	WP_096366939.1	PEP-CTERM sorting domain-containing protein	-
CFK21_RS12320	2673246..2673569	-	324	WP_096366940.1	helix-hairpin-helix domain-containing protein	-
CFK21_RS12325	2673668..2673985	-	318	WP_096366941.1	multidrug efflux SMR transporter	-
CFK21_RS12330	2674069..2674368	-	300	WP_096367609.1	HigA family addiction module antidote protein	Antitoxin
CFK21_RS12335	2674380..2674661	-	282	WP_096366942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFK21_RS15305	2674831..2675190	-	360	WP_157745665.1	hypothetical protein	-
CFK21_RS12340	2675277..2676131	-	855	WP_157745667.1	IS3 family transposase	-
CFK21_RS12345	2676128..2676499	-	372	WP_096366944.1	transposase	-
CFK21_RS12350	2676608..2677795	-	1188	Protein_2463	IS3 family transposase	-
CFK21_RS12355	2677868..2678518	-	651	WP_096366945.1	DUF4145 domain-containing protein	-
CFK21_RS12360	2678615..2679352	-	738	WP_096366946.1	adenylosuccinate lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	2674004..2687279	13275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10792.55 Da
• Isoelectric Point: 10.1313

>T31693 WP_096366942.1 NZ_AP018052:c2674661-2674380 [Thiohalobacter thiocyanaticus]
MIKSFKGKEAEKIYQGCYSKRIPKDIQRLAARKLEMLAAASDLKSLRIPPSNRLEKLKGDRAGQYSIRINDQWRICFIWK
AGDAHEVDIVDYH

Nucleotide

• Length: 282 bp

>T31693 NZ_AP018052:c2674661-2674380 [Thiohalobacter thiocyanaticus]
ATGATCAAATCGTTCAAGGGCAAGGAAGCTGAGAAGATTTATCAGGGGTGCTATTCGAAGCGGATTCCCAAAGACATTCA
ACGCCTTGCGGCACGGAAACTGGAAATGCTCGCTGCGGCGAGCGATCTCAAATCATTGAGAATTCCACCGTCCAACCGTC
TTGAAAAACTGAAGGGTGACAGGGCAGGTCAATACAGTATACGAATCAACGATCAATGGCGTATCTGCTTTATCTGGAAA
GCCGGTGATGCTCATGAGGTGGATATCGTTGATTATCATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11378.12 Da
• Isoelectric Point: 8.0293

>AT31693 WP_096367609.1 NZ_AP018052:c2674368-2674069 [Thiohalobacter thiocyanaticus]
MAKRDFDPIHPGEILLEEFLRPMGISQYRLAKDISVAQRRISEITQGKRSITADTALRLGRYFGMEAQFWLNLQSRYDLL
CAEAELAKRLDKEVTPHAA

Nucleotide

• Length: 300 bp

>AT31693 NZ_AP018052:c2674368-2674069 [Thiohalobacter thiocyanaticus]
ATGGCTAAGAGAGATTTCGATCCCATTCATCCTGGTGAAATTCTGCTTGAGGAATTCTTGCGGCCCATGGGTATATCTCA
ATACCGCCTTGCAAAAGATATCAGTGTGGCGCAACGCCGTATAAGCGAAATTACCCAAGGCAAGCGGTCTATTACAGCTG
ATACGGCGTTGCGCCTCGGTCGGTACTTTGGCATGGAGGCGCAGTTTTGGCTCAATCTTCAGTCGCGTTACGATCTTTTG
TGTGCAGAAGCGGAGCTCGCCAAACGGCTGGACAAAGAAGTTACGCCCCATGCGGCATAG