Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3542268..3542938 Replicon chromosome
Accession NZ_AP018036
Organism Mycobacterium tuberculosis strain HN-506

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CPZ04_RS17355 Protein ID WP_003899954.1
Coordinates 3542268..3542612 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CPZ04_RS17360 Protein ID WP_003899955.1
Coordinates 3542609..3542938 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ04_RS17330 3537573..3537866 + 294 WP_003416635.1 hypothetical protein -
CPZ04_RS17335 3538154..3539443 + 1290 WP_003416640.1 ATP-binding protein -
CPZ04_RS17345 3541148..3541582 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CPZ04_RS17350 3541585..3542037 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CPZ04_RS17355 3542268..3542612 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ04_RS17360 3542609..3542938 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CPZ04_RS17365 3543476..3543823 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CPZ04_RS17370 3543820..3544440 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
CPZ04_RS17375 3544600..3545865 - 1266 WP_003902423.1 hypothetical protein -
CPZ04_RS17380 3546033..3546242 + 210 WP_003416778.1 hypothetical protein -
CPZ04_RS17390 3546489..3547523 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T31684 WP_003899954.1 NZ_AP018036:3542268-3542612 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T31684 NZ_AP018036:3542268-3542612 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT31684 WP_003899955.1 NZ_AP018036:3542609-3542938 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT31684 NZ_AP018036:3542609-3542938 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References