Detailed information of TA system TA031624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3550712..3551382
• Replicon: chromosome
• Accession: NZ_AP018035
• Organism: Mycobacterium tuberculosis strain HN-321

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: CPZ12_RS17385
• Protein ID: WP_003899954.1
• Coordinates: 3550712..3551056 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: CPZ12_RS17390
• Protein ID: WP_003899955.1
• Coordinates: 3551053..3551382 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ12_RS17350	3545785..3546645	+	861	WP_003416628.1	alpha/beta hydrolase	-
CPZ12_RS17355	3546620..3547135	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
CPZ12_RS17365	3547375..3547668	+	294	WP_003416635.1	hypothetical protein	-
CPZ12_RS17370	3547956..3549245	+	1290	WP_003416640.1	ATP-binding protein	-
CPZ12_RS17375	3549592..3550026	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
CPZ12_RS17380	3550029..3550481	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CPZ12_RS17385	3550712..3551056	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPZ12_RS17390	3551053..3551382	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
CPZ12_RS17395	3551920..3552267	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
CPZ12_RS17400	3552264..3552884	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
CPZ12_RS17405	3553044..3554309	-	1266	WP_003902423.1	hypothetical protein	-
CPZ12_RS17410	3554477..3554686	+	210	WP_003416778.1	hypothetical protein	-
CPZ12_RS17420	3554933..3555967	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T31624 WP_003899954.1 NZ_AP018035:3550712-3551056 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T31624 NZ_AP018035:3550712-3551056 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT31624 WP_003899955.1 NZ_AP018035:3551053-3551382 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT31624 NZ_AP018035:3551053-3551382 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6