Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3539535..3540205 Replicon chromosome
Accession NZ_AP018033
Organism Mycobacterium tuberculosis strain HN-024

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CPZ07_RS17210 Protein ID WP_003899954.1
Coordinates 3539535..3539879 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CPZ07_RS17215 Protein ID WP_003899955.1
Coordinates 3539876..3540205 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ07_RS17175 3534608..3535468 + 861 WP_003416628.1 alpha/beta hydrolase -
CPZ07_RS17180 3535443..3535958 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
CPZ07_RS17190 3536198..3536491 + 294 WP_003416635.1 hypothetical protein -
CPZ07_RS17195 3536779..3538068 + 1290 WP_003416640.1 ATP-binding protein -
CPZ07_RS17200 3538415..3538849 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CPZ07_RS17205 3538852..3539304 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CPZ07_RS17210 3539535..3539879 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ07_RS17215 3539876..3540205 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CPZ07_RS17220 3540743..3541090 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CPZ07_RS17225 3541087..3541707 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
CPZ07_RS17230 3541867..3543132 - 1266 WP_003909837.1 hypothetical protein -
CPZ07_RS17235 3543300..3543509 + 210 WP_003416778.1 hypothetical protein -
CPZ07_RS17245 3543756..3544790 - 1035 WP_003905011.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T31504 WP_003899954.1 NZ_AP018033:3539535-3539879 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T31504 NZ_AP018033:3539535-3539879 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT31504 WP_003899955.1 NZ_AP018033:3539876-3540205 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT31504 NZ_AP018033:3539876-3540205 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References