Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 1533696..1534216 Replicon chromosome
Accession NZ_AP017973
Organism Limosilactobacillus fermentum strain MTCC 25067

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag LF25067_RS07710 Protein ID WP_096494515.1
Coordinates 1533696..1533965 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag LF25067_RS07715 Protein ID WP_096494517.1
Coordinates 1533962..1534216 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LF25067_RS07690 (LF25067_01600) 1528939..1530189 - 1251 WP_096494509.1 RNA-guided endonuclease TnpB family protein -
LF25067_RS07695 1530260..1530718 + 459 Protein_1474 IS200/IS605 family transposase -
LF25067_RS07700 (LF25067_01601) 1530925..1531644 + 720 WP_096494511.1 hypothetical protein -
LF25067_RS07705 (LF25067_01602) 1532133..1533254 + 1122 WP_096494513.1 PTS sugar transporter subunit IIC -
LF25067_RS07710 (LF25067_01603) 1533696..1533965 - 270 WP_096494515.1 Txe/YoeB family addiction module toxin Toxin
LF25067_RS07715 (LF25067_01604) 1533962..1534216 - 255 WP_096494517.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LF25067_RS07720 (LF25067_01605) 1534365..1535030 - 666 Protein_1479 family 1 glycosylhydrolase -
LF25067_RS07725 (LF25067_01606) 1535281..1535940 - 660 WP_096494521.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
LF25067_RS10730 (LF25067_01607) 1536597..1536821 - 225 WP_243685619.1 hypothetical protein -
LF25067_RS07735 (LF25067_01608) 1536826..1537182 - 357 WP_096494523.1 MmcQ/YjbR family DNA-binding protein -
LF25067_RS07740 (LF25067_01609) 1537184..1537789 - 606 WP_243685620.1 sel1 repeat family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1528939..1544188 15249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-55)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10581.36 Da        Isoelectric Point: 10.3034

>T31444 WP_096494515.1 NZ_AP017973:c1533965-1533696 [Limosilactobacillus fermentum]
MSYAIKLKRSANKDLKKIKGSYLEKSFLQIIEQLRKDPLAPNQGFEKLVPPIKGFYSRRINIQYRLVYKVDQDTRTVIIY
SAWSHYEEL

Download         Length: 270 bp

>T31444 NZ_AP017973:c1533965-1533696 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCTTTGGCACCTAACCAAGGCTTTGAGAAATTAGTACCGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGTACCGACTCGTCTATAAGGTTGATCAAGACACGCGAACTGTGATCATCTAT
TCTGCGTGGTCACATTACGAAGAACTGTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9789.09 Da        Isoelectric Point: 4.4237

>AT31444 WP_096494517.1 NZ_AP017973:c1534216-1533962 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPIVIKPTKADEKSVVVISEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Download         Length: 255 bp

>AT31444 NZ_AP017973:c1534216-1533962 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GATTGTGATTAAGCCGACCAAGGCGGATGAAAAGAGCGTTGTCGTCATTAGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References