Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2962517..2963165 Replicon chromosome
Accession NZ_AP017901
Organism Mycobacterium tuberculosis strain NCGM946K2

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag CPZ08_RS14425 Protein ID WP_003899414.1
Coordinates 2962517..2962840 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag CPZ08_RS14430 Protein ID WP_003899415.1
Coordinates 2962920..2963165 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ08_RS14405 2958178..2959439 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CPZ08_RS14410 2959726..2961165 - 1440 WP_003899411.1 phage major capsid protein -
CPZ08_RS14415 2961173..2961706 - 534 WP_003899412.1 HK97 family phage prohead protease -
CPZ08_RS14420 2961859..2962350 - 492 WP_003900541.1 phage terminase small subunit P27 family -
CPZ08_RS14425 2962517..2962840 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
CPZ08_RS14430 2962920..2963165 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
CPZ08_RS14435 2963162..2964589 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
CPZ08_RS14440 2964591..2964983 - 393 WP_003899417.1 DUF2742 domain-containing protein -
CPZ08_RS14445 2964980..2965240 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
CPZ08_RS14450 2965257..2965619 - 363 WP_003900543.1 hypothetical protein -
CPZ08_RS14455 2965622..2966749 - 1128 WP_003899420.1 site-specific integrase -
CPZ08_RS14460 2966894..2967121 - 228 WP_003899421.1 hypothetical protein -
CPZ08_RS14465 2967118..2967507 - 390 WP_003899422.1 hypothetical protein -
CPZ08_RS14470 2967413..2967685 + 273 WP_003900544.1 hypothetical protein -
CPZ08_RS14475 2967784..2968017 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2956482..2966749 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T31408 WP_003899414.1 NZ_AP017901:c2962840-2962517 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T31408 NZ_AP017901:c2962840-2962517 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT31408 WP_003899415.1 NZ_AP017901:c2963165-2962920 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT31408 NZ_AP017901:c2963165-2962920 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References