Detailed information of TA system TA031359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 120190..120707
• Replicon: plasmid pSFD01
• Accession: NZ_AP017899
• Organism: Sphingopyxis sp. FD7

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: SPYCA_RS18420
• Protein ID: WP_081095537.1
• Coordinates: 120441..120707 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A2Z5USK1
• Locus tag: SPYCA_RS18415
• Protein ID: WP_067403131.1
• Coordinates: 120190..120444 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPYCA_RS18390	117242..118192	+	951	WP_120222507.1	DUF1738 domain-containing protein	-
SPYCA_RS18400	118601..119242	+	642	WP_054590614.1	hypothetical protein	-
SPYCA_RS18405	119255..119878	+	624	WP_120222509.1	hypothetical protein	-
SPYCA_RS18410	119895..120095	+	201	WP_120222510.1	hypothetical protein	-
SPYCA_RS18415	120190..120444	+	255	WP_067403131.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SPYCA_RS18420	120441..120707	+	267	WP_081095537.1	Txe/YoeB family addiction module toxin	Toxin
SPYCA_RS18425	120722..121063	+	342	WP_120222511.1	hypothetical protein	-
SPYCA_RS18430	121087..121584	-	498	WP_120222512.1	hypothetical protein	-
SPYCA_RS18435	121652..122938	+	1287	WP_120222513.1	hypothetical protein	-
SPYCA_RS18440	123079..123621	+	543	WP_146625187.1	hypothetical protein	-
SPYCA_RS18445	123724..124248	+	525	WP_120222515.1	hypothetical protein	-
SPYCA_RS18450	124274..125119	-	846	WP_053554354.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..242612	242612
	-	flank	IS/Tn	-	-	124274..125119	845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10423.88 Da
• Isoelectric Point: 9.0994

>T31359 WP_081095537.1 NZ_AP017899:120441-120707 [Sphingopyxis sp. FD7]
MKLVFADNAWEDYLHWQQADDKVLTRLNDLIKECQRTPFKGTGKPEPLKGSLAGWWSRRITKEDRLVYRVTGKGNEQQLE
IAQCRFHY

Nucleotide

• Length: 267 bp

>T31359 NZ_AP017899:120441-120707 [Sphingopyxis sp. FD7]
TTGAAGCTGGTCTTTGCTGACAATGCCTGGGAGGATTATCTTCACTGGCAACAAGCCGATGACAAGGTTCTCACCCGGCT
CAACGATCTCATCAAGGAATGCCAGCGAACACCTTTCAAAGGGACCGGAAAGCCTGAGCCTCTCAAAGGCTCCCTGGCAG
GCTGGTGGTCGCGGCGGATCACCAAAGAAGACCGTCTGGTCTATCGCGTCACTGGCAAAGGAAACGAGCAACAGCTAGAG
ATTGCCCAATGCCGGTTTCACTATTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9506.68 Da
• Isoelectric Point: 4.4354

>AT31359 WP_067403131.1 NZ_AP017899:120190-120444 [Sphingopyxis sp. FD7]
MDVITYSEARANLKGIMDQVVSDRTHVVVTRQKAESIVMVSLEDWNAIEETMYLLSNRTNADRLRQSIEQLDGQGGNERG
LIEP

Nucleotide

• Length: 255 bp

>AT31359 NZ_AP017899:120190-120444 [Sphingopyxis sp. FD7]
ATGGATGTTATCACCTACTCAGAAGCGCGCGCCAATCTGAAAGGCATTATGGATCAAGTGGTCAGTGACCGGACACATGT
CGTGGTCACGCGCCAGAAAGCAGAGTCGATCGTGATGGTCTCGCTCGAAGATTGGAACGCGATCGAGGAGACCATGTACC
TCCTTTCGAACCGCACCAATGCCGACCGCCTACGGCAGTCGATCGAGCAACTGGATGGTCAAGGCGGCAATGAGCGCGGG
CTGATCGAGCCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2Z5USK1