31328

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1032219..1032837 Replicon chromosome
Accession NZ_AP017655
Organism Sphingobium cloacae strain JCM 10874

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag SCLO_RS05075 Protein ID WP_066521714.1
Coordinates 1032541..1032837 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag SCLO_RS05070 Protein ID WP_066521712.1
Coordinates 1032219..1032527 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SCLO_RS05055 1027462..1028976 - 1515 WP_096362065.1 IS21 family transposase -
SCLO_RS05060 1029089..1030360 - 1272 WP_066522486.1 integrase arm-type DNA-binding domain-containing protein -
SCLO_RS23250 1030353..1030499 - 147 WP_157080368.1 hypothetical protein -
SCLO_RS05065 1030496..1032202 - 1707 WP_096362092.1 recombinase family protein -
SCLO_RS05070 1032219..1032527 - 309 WP_066521712.1 HigA family addiction module antidote protein Antitoxin
SCLO_RS05075 1032541..1032837 - 297 WP_066521714.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SCLO_RS05080 1032919..1033137 - 219 WP_066521717.1 hypothetical protein -
SCLO_RS05085 1033146..1033358 - 213 WP_066521719.1 AlpA family transcriptional regulator -
SCLO_RS05090 1033527..1033991 - 465 WP_066521722.1 hypothetical protein -
SCLO_RS05095 1034228..1034899 + 672 WP_157080345.1 hypothetical protein -
SCLO_RS05100 1034981..1035781 + 801 WP_123905447.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1020761..1049613 28852


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11599.96 Da        Isoelectric Point: 5.9695

>T31328 WP_066521714.1 NZ_AP017655:c1032837-1032541 [Sphingobium cloacae]
MIVGFRDDWLRAFFVDDVHSRNIPSDLEARLFRKLQMIDDAVSDSDLRVPPSNHFEKLRGNLAGFHSIRVNQQWRLIFRW
DGEHGEADGIYLDDHSYR

Download         Length: 297 bp

>T31328 NZ_AP017655:c1032837-1032541 [Sphingobium cloacae]
ATGATCGTCGGCTTTCGGGATGACTGGCTGCGGGCCTTTTTCGTGGATGATGTCCACTCCCGCAATATCCCGTCCGACCT
CGAAGCCCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGTATCCGATTCGGACCTTCGCGTGCCGCCCAGTAATC
ATTTCGAGAAGCTGCGCGGCAATCTGGCGGGGTTTCATTCGATCCGTGTCAACCAGCAATGGCGGTTGATCTTCCGCTGG
GACGGGGAGCACGGAGAGGCGGACGGGATCTATCTGGACGACCACAGCTACCGATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11671.55 Da        Isoelectric Point: 10.7180

>AT31328 WP_066521712.1 NZ_AP017655:c1032527-1032219 [Sphingobium cloacae]
MLMTKRKPASVGEILTEEFMEPMGLTQGALAEAMGVQRKHVNELCGNRRNITAATALILARVFGNTPDFWLNLQRRNDLW
EVMNTPRERERVERARPLKHAA

Download         Length: 309 bp

>AT31328 NZ_AP017655:c1032527-1032219 [Sphingobium cloacae]
ATGCTGATGACTAAGCGCAAGCCGGCCTCTGTCGGCGAAATCCTGACCGAGGAATTCATGGAGCCGATGGGGCTGACCCA
GGGCGCCTTGGCCGAAGCGATGGGCGTGCAGCGCAAGCACGTCAACGAACTGTGCGGAAACCGCCGGAACATCACCGCTG
CGACCGCGCTCATTCTTGCCCGCGTATTCGGCAACACTCCCGACTTCTGGCTCAACCTCCAGCGGCGCAACGACCTGTGG
GAAGTGATGAACACGCCAAGGGAACGTGAGCGGGTCGAGCGTGCCCGCCCGTTGAAGCACGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References