Detailed information of TA system TA031295

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41538..41771
• Replicon: plasmid pMRY15-131_1
• Accession: NZ_AP017621
• Organism: Escherichia coli strain MRY15-131

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CPY99_RS25815
• Protein ID: WP_001312861.1
• Coordinates: 41538..41696 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41740..41771 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPY99_RS25775	36585..36812	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
CPY99_RS25780	36906..37592	-	687	WP_000332484.1	PAS domain-containing protein	-
CPY99_RS25785	37783..38166	-	384	WP_000124981.1	relaxosome protein TraM	-
CPY99_RS25790	38443..39090	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
CPY99_RS25795	39387..40208	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CPY99_RS25800	40329..40616	-	288	WP_000107535.1	hypothetical protein	-
CPY99_RS25805	40641..40847	-	207	WP_000547939.1	hypothetical protein	-
CPY99_RS25815	41538..41696	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41740..41771	-	32	NuclAT_1	-	Antitoxin
-	41740..41771	-	32	NuclAT_1	-	Antitoxin
-	41740..41771	-	32	NuclAT_1	-	Antitoxin
-	41740..41771	-	32	NuclAT_1	-	Antitoxin
-	43213..43410	-	198	NuclAT_0	-	-
-	43213..43410	-	198	NuclAT_0	-	-
-	43213..43410	-	198	NuclAT_0	-	-
-	43213..43410	-	198	NuclAT_0	-	-
CPY99_RS27260	43222..43410	+	189	WP_001299721.1	hypothetical protein	-
CPY99_RS25825	43422..44141	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
CPY99_RS25830	44138..44572	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
CPY99_RS25835	44627..46585	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / mph(A) / sitABCD / erm(B) / aac(3)-IIa / blaCTX-M-27	-	1..116529	116529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T31295 WP_001312861.1 NZ_AP017621:c41696-41538 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T31295 NZ_AP017621:c41696-41538 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT31295 NZ_AP017621:c41771-41740 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin