31268

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1090783..1091285 Replicon chromosome
Accession NZ_AP017620
Organism Escherichia coli strain MRY15-131

Toxin (Protein)


Gene name yoeB Uniprot ID V0THH9
Locus tag CPY99_RS05550 Protein ID WP_023141665.1
Coordinates 1091031..1091285 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CPY99_RS05545 Protein ID WP_001259255.1
Coordinates 1090783..1091034 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPY99_RS05515 1085961..1087028 - 1068 WP_000080125.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CPY99_RS05520 1087028..1088098 - 1071 WP_000108989.1 histidinol-phosphate transaminase -
CPY99_RS05525 1088095..1089399 - 1305 WP_024175832.1 histidinol dehydrogenase -
CPY99_RS05530 1089405..1090304 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CPY99_RS05540 1090450..1090500 - 51 WP_001364200.1 his operon leader peptide -
CPY99_RS05545 1090783..1091034 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CPY99_RS05550 1091031..1091285 + 255 WP_023141665.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CPY99_RS05555 1091368..1092192 + 825 WP_000754758.1 SDR family oxidoreductase -
CPY99_RS05560 1092238..1093167 + 930 WP_000803351.1 LysR family transcriptional regulator -
CPY99_RS26850 1093382..1093444 + 63 WP_010723108.1 membrane protein YoeI -
CPY99_RS05565 1093434..1094792 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CPY99_RS05570 1094971..1096029 + 1059 WP_000492324.1 transport protein YeeE -
CPY99_RS05575 1096043..1096270 + 228 WP_061092870.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10229.67 Da        Isoelectric Point: 7.8733

>T31268 WP_023141665.1 NZ_AP017620:1091031-1091285 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFECKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T31268 NZ_AP017620:1091031-1091285 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAATGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT31268 WP_001259255.1 NZ_AP017620:1090783-1091034 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT31268 NZ_AP017620:1090783-1091034 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0THH9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References