31238

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1723032..1723594 Replicon chromosome
Accession NZ_AP017617
Organism Escherichia coli strain MRY15-117

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag CPZ03_RS08780 Protein ID WP_000605676.1
Coordinates 1723032..1723310 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CPZ03_RS08785 Protein ID WP_000781370.1
Coordinates 1723310..1723594 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ03_RS08750 1718618..1719049 - 432 WP_000152305.1 peroxiredoxin OsmC -
CPZ03_RS08760 1719394..1719609 + 216 WP_000495766.1 biofilm-dependent modulation protein -
CPZ03_RS08765 1719711..1719848 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CPZ03_RS08770 1720005..1721702 + 1698 WP_000433464.1 malate dehydrogenase -
CPZ03_RS08775 1721836..1722846 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
CPZ03_RS08780 1723032..1723310 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ03_RS08785 1723310..1723594 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
CPZ03_RS08790 1723645..1724298 - 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
CPZ03_RS08795 1724291..1725175 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
CPZ03_RS08800 1725188..1728235 - 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T31238 WP_000605676.1 NZ_AP017617:1723032-1723310 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T31238 NZ_AP017617:1723032-1723310 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT31238 WP_000781370.1 NZ_AP017617:1723310-1723594 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT31238 NZ_AP017617:1723310-1723594 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References