Detailed information of TA system TA031219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127261..127687
• Replicon: plasmid pMRY16-002_1
• Accession: NZ_AP017611
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CPZ02_RS26160
• Protein ID: WP_001312861.1
• Coordinates: 127261..127419 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	127463..127687 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS26120	122279..122674	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
CPZ02_RS26125	122773..123462	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
CPZ02_RS26130	123649..124032	-	384	WP_001151524.1	relaxosome protein TraM	-
CPZ02_RS26135	124353..124955	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
CPZ02_RS26140	125252..126073	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CPZ02_RS26145	126198..126485	-	288	WP_000107535.1	hypothetical protein	-
CPZ02_RS26160	127261..127419	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	127463..127687	-	225	NuclAT_0	-	Antitoxin
-	127463..127687	-	225	NuclAT_0	-	Antitoxin
-	127463..127687	-	225	NuclAT_0	-	Antitoxin
-	127463..127687	-	225	NuclAT_0	-	Antitoxin
CPZ02_RS26165	127656..128417	-	762	Protein_122	plasmid SOS inhibition protein A	-
CPZ02_RS26170	128414..128848	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
CPZ02_RS26175	128903..130861	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
CPZ02_RS26180	130920..131153	-	234	WP_000006004.1	DUF905 domain-containing protein	-
CPZ02_RS26185	131209..131736	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
CPZ02_RS26205	132209..132451	-	243	WP_096488219.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / espC / iutA / iucD / iucC / iucB / iucA	1..168972	168972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T31219 WP_001312861.1 NZ_AP017611:c127419-127261 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T31219 NZ_AP017611:c127419-127261 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT31219 NZ_AP017611:c127687-127463 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin