Detailed information of TA system TA031214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4343951..4344553
• Replicon: chromosome
• Accession: NZ_AP017610
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CPZ02_RS22390
• Protein ID: WP_000897305.1
• Coordinates: 4343951..4344262 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CPZ02_RS22395
• Protein ID: WP_000356397.1
• Coordinates: 4344263..4344553 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS22365	4339865..4340464	+	600	WP_001315111.1	glucose-1-phosphatase	-
CPZ02_RS22370	4340458..4341330	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CPZ02_RS22375	4341327..4341764	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CPZ02_RS22380	4341809..4342750	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CPZ02_RS22385	4342814..4343722	-	909	WP_001318161.1	alpha/beta hydrolase	-
CPZ02_RS22390	4343951..4344262	+	312	WP_000897305.1	hypothetical protein	Toxin
CPZ02_RS22395	4344263..4344553	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CPZ02_RS22400	4345158..4345376	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CPZ02_RS22405	4345591..4346520	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CPZ02_RS22410	4346517..4347152	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CPZ02_RS22415	4347149..4348051	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T31214 WP_000897305.1 NZ_AP017610:4343951-4344262 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T31214 NZ_AP017610:4343951-4344262 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT31214 WP_000356397.1 NZ_AP017610:4344263-4344553 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT31214 NZ_AP017610:4344263-4344553 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7