Detailed information of TA system TA031212

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3959792..3960049
• Replicon: chromosome
• Accession: NZ_AP017610
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: hokA
• Uniprot ID: A0A0L6NKI3
• Locus tag: CPZ02_RS20500
• Protein ID: WP_001135743.1
• Coordinates: 3959792..3959944 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokA
Locus tag	-
Coordinates	3959995..3960049 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS20475	3955891..3956549	+	659	Protein_3754	OmpA family lipoprotein	-
CPZ02_RS20480	3956653..3957627	+	975	WP_000805008.1	glyoxylate/hydroxypyruvate reductase GhrB	-
CPZ02_RS20485	3957677..3958387	-	711	WP_000190516.1	DUF3053 domain-containing protein	-
CPZ02_RS20490	3958821..3959111	+	291	WP_000455798.1	HTH-type transcriptional regulator	-
CPZ02_RS20495	3959392..3959604	+	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
CPZ02_RS20500	3959792..3959944	-	153	WP_001135743.1	type I toxin-antitoxin system toxin HokA	Toxin
-	3959995..3960049	+	55	-	-	Antitoxin
CPZ02_RS20505	3960266..3962335	-	2070	WP_001291790.1	glycine--tRNA ligase subunit beta	-
CPZ02_RS20510	3962345..3963256	-	912	WP_001168544.1	glycine--tRNA ligase subunit alpha	-
CPZ02_RS20515	3963351..3963659	-	309	WP_000980157.1	YsaB family lipoprotein	-
CPZ02_RS20520	3963834..3964829	+	996	WP_001182665.1	O-acetyltransferase WecH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5898.11 Da
• Isoelectric Point: 7.7173

>T31212 WP_001135743.1 NZ_AP017610:c3959944-3959792 [Escherichia coli]
MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFLACNLKE

Nucleotide

• Length: 153 bp

>T31212 NZ_AP017610:c3959944-3959792 [Escherichia coli]
ATGCCGCAGAAATATAGATTGCTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTTACCTGGATGGTAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGGAAGTTATGAGCTGGCGGCATTTTTAGCCTGCAATTTGAAAGAGTAA

Antitoxin

Nucleotide

• Length: 55 bp

>AT31212 NZ_AP017610:3959995-3960049 [Escherichia coli]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Structures

Toxin

• AlphaFold DB: A0A0L6NKI3

Antitoxin