Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 102979..103588 Replicon plasmid ABRG53f
Accession NZ_AP017566
Organism Pseudanabaena sp. ABRG5-3

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag ABRG53_RS25210 Protein ID WP_126391737.1
Coordinates 103304..103588 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ABRG53_RS25205 Protein ID WP_103668857.1
Coordinates 102979..103290 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABRG53_RS25180 98784..99896 + 1113 WP_126391727.1 glycosyltransferase family 4 protein -
ABRG53_RS25185 99900..100790 + 891 WP_126391729.1 NAD-dependent epimerase/dehydratase family protein -
ABRG53_RS25190 100800..101738 + 939 WP_126391731.1 hypothetical protein -
ABRG53_RS25195 101825..102766 + 942 WP_126391733.1 NAD-dependent epimerase/dehydratase family protein -
ABRG53_RS25200 102777..102956 + 180 WP_126391735.1 hypothetical protein -
ABRG53_RS25205 102979..103290 - 312 WP_103668857.1 HigA family addiction module antidote protein Antitoxin
ABRG53_RS25210 103304..103588 - 285 WP_126391737.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ABRG53_RS25215 104007..105500 + 1494 WP_162615740.1 pentapeptide repeat-containing protein -
ABRG53_RS25220 105648..106289 - 642 WP_126391741.1 restriction endonuclease subunit R -
ABRG53_RS25225 106566..106895 - 330 WP_126391743.1 type II toxin-antitoxin system PemK/MazF family toxin -
ABRG53_RS25230 106882..107088 - 207 WP_126391745.1 hypothetical protein -
ABRG53_RS25235 107129..107329 - 201 WP_126391747.1 hypothetical protein -
ABRG53_RS25240 107314..107916 + 603 WP_126391749.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110608 110608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(9-64)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11171.69 Da        Isoelectric Point: 9.4454

>T31160 WP_126391737.1 NZ_AP017566:c103588-103304 [Pseudanabaena sp. ABRG5-3]
MPQKYKDKRTAKFAAGDRVKEFQGFERQAYKRLEILEAAPNKEALMALPSNRFEALGGDRKGQYSIRINDKWRICFEWTE
TENRPFNIEIVDYH

Download         Length: 285 bp

>T31160 NZ_AP017566:c103588-103304 [Pseudanabaena sp. ABRG5-3]
ATGCCTCAAAAATATAAAGACAAACGTACAGCCAAATTTGCGGCTGGCGATCGTGTCAAAGAATTTCAAGGCTTTGAAAG
GCAAGCATACAAACGTCTAGAAATTCTAGAAGCCGCACCTAATAAAGAAGCTCTTATGGCATTGCCAAGTAACCGTTTTG
AGGCACTAGGCGGAGATAGAAAAGGACAGTACAGCATCCGCATAAATGACAAATGGCGTATTTGTTTTGAGTGGACAGAA
ACAGAAAATCGCCCATTTAATATCGAAATTGTCGATTATCACTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11429.02 Da        Isoelectric Point: 5.7152

>AT31160 WP_103668857.1 NZ_AP017566:c103290-102979 [Pseudanabaena sp. ABRG5-3]
MARPAIHAGEILADELEELGISASELARSLHIPTNRITQILKGQRGITADTALRLGRWFGTGAELWLNLQKAYELRLAEE
LAGEEIQKTIQPRSSNNQQLVQV

Download         Length: 312 bp

>AT31160 NZ_AP017566:c103290-102979 [Pseudanabaena sp. ABRG5-3]
ATGGCAAGACCTGCAATTCACGCTGGCGAAATCTTAGCAGATGAGCTAGAAGAACTGGGAATTAGTGCATCAGAACTAGC
GCGATCGCTCCATATCCCCACAAATCGGATTACGCAAATTCTCAAAGGACAAAGAGGTATTACTGCTGACACGGCACTAC
GTCTAGGACGATGGTTTGGCACTGGTGCAGAGCTATGGCTTAATTTGCAAAAAGCCTATGAGTTACGCCTAGCCGAAGAG
TTGGCGGGAGAAGAAATTCAAAAAACAATTCAACCTCGCTCATCAAACAATCAGCAGCTAGTTCAAGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References