Detailed information of TA system TA031152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1106253..1106848
• Replicon: chromosome
• Accession: NZ_AP017560
• Organism: Pseudanabaena sp. ABRG5-3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: ABRG53_RS05075
• Protein ID: WP_126385627.1
• Coordinates: 1106567..1106848 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABRG53_RS05070
• Protein ID: WP_126385626.1
• Coordinates: 1106253..1106555 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABRG53_RS05020	1101369..1101632	-	264	WP_126385618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ABRG53_RS05025	1101782..1102235	-	454	Protein_999	HNH endonuclease	-
ABRG53_RS05030	1102243..1102482	-	240	WP_126385619.1	hypothetical protein	-
ABRG53_RS05035	1102624..1103180	-	557	Protein_1001	DUF3782 domain-containing protein	-
ABRG53_RS05040	1103414..1103719	+	306	WP_126385620.1	helix-turn-helix domain-containing protein	-
ABRG53_RS05045	1103725..1104078	-	354	WP_126385621.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ABRG53_RS05050	1104075..1104590	-	516	WP_126385622.1	DUF2726 domain-containing protein	-
ABRG53_RS05055	1104626..1105435	-	810	WP_126385623.1	hypothetical protein	-
ABRG53_RS05060	1105581..1105901	-	321	WP_126385624.1	RNA-binding protein	-
ABRG53_RS05065	1105901..1106122	-	222	WP_126385625.1	type II toxin-antitoxin system HicB family antitoxin	-
ABRG53_RS05070	1106253..1106555	-	303	WP_126385626.1	HigA family addiction module antidote protein	Antitoxin
ABRG53_RS05075	1106567..1106848	-	282	WP_126385627.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABRG53_RS05080	1106928..1107551	+	624	WP_126385628.1	hypothetical protein	-
ABRG53_RS05085	1107633..1108574	+	942	WP_126385629.1	methionyl-tRNA formyltransferase	-
ABRG53_RS05090	1108714..1111386	+	2673	WP_126385630.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10886.49 Da
• Isoelectric Point: 8.5073

>T31152 WP_126385627.1 NZ_AP017560:c1106848-1106567 [Pseudanabaena sp. ABRG5-3]
MIGSFACKETEKIWQGMRSRKLPSDIQERALRKLRQLDVSANLEDLRVPPANHLEALVGDRLGQYSIRITKQWRLCFVWQ
NGNAYDVEIVDYH

Nucleotide

• Length: 282 bp

>T31152 NZ_AP017560:c1106848-1106567 [Pseudanabaena sp. ABRG5-3]
ATGATTGGTTCGTTTGCTTGTAAAGAAACCGAGAAGATTTGGCAAGGGATGCGATCGCGTAAGTTACCATCAGATATACA
GGAAAGGGCTTTACGCAAATTACGTCAACTTGATGTGTCTGCTAATCTGGAAGATCTGCGTGTACCACCTGCCAATCATC
TAGAAGCACTGGTTGGCGATCGCCTTGGACAATACAGTATTCGCATTACGAAGCAGTGGCGACTATGTTTTGTTTGGCAA
AATGGAAATGCTTATGATGTTGAGATTGTTGATTATCATTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11541.28 Da
• Isoelectric Point: 4.7868

>AT31152 WP_126385626.1 NZ_AP017560:c1106555-1106253 [Pseudanabaena sp. ABRG5-3]
MEDTLLRNPHVGEILQYEFLEELDINVNSLADSLRLSYASIQQIIQGKAPMTADVDLRLCRYFGLSDGYFLRLHYAYELM
EAKRNLGDILRQIVPITALH

Nucleotide

• Length: 303 bp

>AT31152 NZ_AP017560:c1106555-1106253 [Pseudanabaena sp. ABRG5-3]
ATGGAAGATACTTTATTGCGTAACCCCCATGTTGGTGAAATCTTGCAATATGAGTTTTTGGAAGAGTTAGATATTAATGT
GAATAGTCTAGCTGATAGTTTGCGGTTGTCATATGCGAGTATTCAGCAAATTATTCAAGGTAAGGCTCCGATGACTGCGG
ATGTGGACTTGCGGTTATGTCGCTATTTTGGATTGTCGGATGGTTATTTTTTGCGGTTGCATTATGCCTATGAGTTGATG
GAAGCAAAAAGAAATTTAGGCGATATATTGCGTCAGATAGTCCCGATTACAGCATTACATTAG