Detailed information of TA system TA031123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1436882..1437541
• Replicon: chromosome
• Accession: NZ_AP017422
• Organism: Filimonas lacunae strain NBRC 104114

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FLA_RS05865
• Protein ID: WP_076380175.1
• Coordinates: 1436882..1437160 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FLA_RS05870
• Protein ID: WP_076380174.1
• Coordinates: 1437203..1437541 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLA_RS05855	1432967..1433365	-	399	WP_144264070.1	hypothetical protein	-
FLA_RS05860	1434125..1436743	+	2619	WP_144264069.1	T9SS type A sorting domain-containing protein	-
FLA_RS05865	1436882..1437160	+	279	WP_076380175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FLA_RS05870	1437203..1437541	+	339	WP_076380174.1	HigA family addiction module antidote protein	Antitoxin
FLA_RS05875	1437746..1440199	-	2454	WP_076380230.1	DUF4982 domain-containing protein	-
FLA_RS05880	1440291..1441022	-	732	WP_076380173.1	LytTR family DNA-binding domain-containing protein	-
FLA_RS05885	1441000..1442070	-	1071	WP_076380172.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10426.04 Da
• Isoelectric Point: 7.2729

>T31123 WP_076380175.1 NZ_AP017422:1436882-1437160 [Filimonas lacunae]
MITGIQHKGLKLLWTKNDGSKLPPAQIGKIRNILVLLDCAVVVEDVNFPGSGLHQLKGDLKGYWSITVTGNYRIFFKFED
GDVYLINYDDYH

Nucleotide

• Length: 279 bp

>T31123 NZ_AP017422:1436882-1437160 [Filimonas lacunae]
ATGATAACAGGCATTCAGCACAAAGGGTTAAAGTTGCTCTGGACCAAAAATGACGGGTCAAAGCTGCCGCCGGCGCAAAT
AGGTAAAATAAGAAACATACTTGTGCTATTAGATTGTGCGGTGGTTGTAGAAGATGTAAATTTTCCCGGGTCGGGGCTGC
ACCAGCTAAAAGGTGATTTGAAAGGATACTGGTCAATTACAGTAACCGGTAACTATCGTATCTTTTTCAAGTTTGAAGAT
GGTGATGTGTACCTGATCAATTACGATGATTATCATTAG

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12233.39 Da
• Isoelectric Point: 10.3761

>AT31123 WP_076380174.1 NZ_AP017422:1437203-1437541 [Filimonas lacunae]
MLKRGMAPVHPGAMLREMYLEPLGLSVTELAGNIGVARRTVSLLINEHSGVSAEMALRLAKAFGTTPELWLNMQQGFDLW
HAGKKVVLKDIHHYSGAKAQTAALEARVMARV

Nucleotide

• Length: 339 bp

>AT31123 NZ_AP017422:1437203-1437541 [Filimonas lacunae]
ATGTTAAAAAGAGGAATGGCACCGGTGCATCCGGGTGCAATGTTAAGAGAAATGTATTTAGAGCCACTGGGGTTAAGTGT
TACCGAGCTGGCCGGCAATATCGGGGTTGCCCGTAGAACGGTTTCTTTGCTTATTAACGAGCATTCGGGTGTAAGCGCAG
AAATGGCGTTGCGTTTGGCAAAGGCTTTTGGAACTACCCCCGAATTGTGGTTGAATATGCAGCAGGGGTTTGATCTGTGG
CATGCAGGGAAGAAGGTGGTGTTGAAGGATATTCATCATTATTCCGGAGCAAAGGCGCAAACGGCAGCTTTGGAAGCACG
TGTGATGGCGAGGGTTTAG