Detailed information of TA system TA031085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2520427..2520944
• Replicon: chromosome
• Accession: NZ_AP017320
• Organism: Staphylococcus aureus strain MI

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: SAMI_RS12925
• Protein ID: WP_000113265.1
• Coordinates: 2520427..2520693 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SAMI_RS12930
• Protein ID: WP_000584499.1
• Coordinates: 2520693..2520944 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMI_RS12900	2515855..2516391	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
SAMI_RS12905	2516625..2517449	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
SAMI_RS12910	2517666..2517848	-	183	WP_000230292.1	hypothetical protein	-
SAMI_RS12915	2517932..2518399	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
SAMI_RS12920	2518585..2520132	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SAMI_RS15495	2520321..2520392	-	72	Protein_2455	hypothetical protein	-
SAMI_RS12925	2520427..2520693	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
SAMI_RS12930	2520693..2520944	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SAMI_RS12935	2521109..2521210	-	102	Protein_2458	hypothetical protein	-
SAMI_RS12940	2521217..2521816	-	600	WP_000162810.1	DsbA family protein	-
SAMI_RS12945	2521835..2522197	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SAMI_RS12950	2522452..2523702	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
SAMI_RS12955	2523799..2524530	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
SAMI_RS12960	2524527..2525246	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T31085 WP_000113265.1 NZ_AP017320:c2520693-2520427 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T31085 NZ_AP017320:c2520693-2520427 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT31085 WP_000584499.1 NZ_AP017320:c2520944-2520693 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT31085 NZ_AP017320:c2520944-2520693 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60