Detailed information of TA system TA031056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5797240..5797835
• Replicon: chromosome
• Accession: NZ_AP017302
• Organism: Pseudomonas aeruginosa strain IOMTU 133

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IOMTU133_RS32885
• Protein ID: WP_079278534.1
• Coordinates: 5797557..5797835 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: IOMTU133_RS26615
• Protein ID: WP_003111575.1
• Coordinates: 5797240..5797545 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOMTU133_RS26580	5792379..5793227	+	849	WP_009878189.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
IOMTU133_RS26590	5793394..5794335	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
IOMTU133_RS26595	5794452..5795066	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
IOMTU133_RS26600	5795108..5795692	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
IOMTU133_RS26605	5795733..5796833	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
IOMTU133_RS26615	5797240..5797545	-	306	WP_003111575.1	HigA family addiction module antidote protein	Antitoxin
IOMTU133_RS32885	5797557..5797835	-	279	WP_079278534.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOMTU133_RS26620	5798164..5800392	+	2229	WP_021264080.1	TonB-dependent receptor	-
IOMTU133_RS26625	5800462..5801109	-	648	WP_003095021.1	carbonate dehydratase	-
IOMTU133_RS26630	5801171..5802409	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10616.17 Da
• Isoelectric Point: 7.8937

>T31056 WP_079278534.1 NZ_AP017302:c5797835-5797557 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWAD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T31056 NZ_AP017302:c5797835-5797557 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGGCGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT31056 WP_003111575.1 NZ_AP017302:c5797545-5797240 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT31056 NZ_AP017302:c5797545-5797240 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCATTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4