Detailed information of TA system TA031055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5084451..5085132
• Replicon: chromosome
• Accession: NZ_AP017302
• Organism: Pseudomonas aeruginosa strain IOMTU 133

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IOMTU133_RS23395
• Protein ID: WP_015503432.1
• Coordinates: 5084767..5085132 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A643IYQ7
• Locus tag: IOMTU133_RS23390
• Protein ID: WP_034071998.1
• Coordinates: 5084451..5084774 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOMTU133_RS23370	5080768..5081406	+	639	WP_003109444.1	hypothetical protein	-
IOMTU133_RS23375	5081649..5081984	+	336	WP_060853238.1	TM2 domain-containing protein	-
IOMTU133_RS23380	5082158..5082676	+	519	WP_003140886.1	PAAR domain-containing protein	-
IOMTU133_RS23385	5082987..5084075	+	1089	WP_003140888.1	DUF3396 domain-containing protein	-
IOMTU133_RS23390	5084451..5084774	-	324	WP_034071998.1	XRE family transcriptional regulator	Antitoxin
IOMTU133_RS23395	5084767..5085132	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOMTU133_RS23400	5085396..5085635	-	240	WP_048521765.1	hypothetical protein	-
IOMTU133_RS23405	5085842..5086114	+	273	WP_003124230.1	hypothetical protein	-
IOMTU133_RS23410	5086145..5086570	-	426	WP_003116492.1	VOC family protein	-
IOMTU133_RS23415	5086671..5087555	+	885	WP_003140892.1	LysR family transcriptional regulator	-
IOMTU133_RS23420	5087528..5088481	-	954	WP_003085661.1	LysR family transcriptional regulator	-
IOMTU133_RS23425	5088702..5089136	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5078467..5087555	9088

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T31055 WP_015503432.1 NZ_AP017302:c5085132-5084767 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T31055 NZ_AP017302:c5085132-5084767 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11926.76 Da
• Isoelectric Point: 6.4751

>AT31055 WP_034071998.1 NZ_AP017302:c5084774-5084451 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEETGA

Nucleotide

• Length: 324 bp

>AT31055 NZ_AP017302:c5084774-5084451 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCATCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGTAACTTCGCTGATATCGAAGAGACGGGCGC
CTAA

Structures

Antitoxin

• AlphaFold DB: A0A643IYQ7