Detailed information of TA system TA031034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2455056..2455663
• Replicon: chromosome
• Accession: NZ_AP014946
• Organism: Variibacter gotjawalensis strain GJW-30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GJW30_RS11915
• Protein ID: WP_096355573.1
• Coordinates: 2455379..2455663 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJW30_RS11910
• Protein ID: WP_096355571.1
• Coordinates: 2455056..2455358 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJW30_RS11895	2452077..2453156	-	1080	WP_096355564.1	NAD(P)-dependent alcohol dehydrogenase	-
GJW30_RS11900	2453201..2453854	-	654	WP_096355566.1	TetR/AcrR family transcriptional regulator	-
GJW30_RS11905	2453910..2454764	-	855	WP_096355569.1	crotonase/enoyl-CoA hydratase family protein	-
GJW30_RS11910	2455056..2455358	-	303	WP_096355571.1	HigA family addiction module antidote protein	Antitoxin
GJW30_RS11915	2455379..2455663	-	285	WP_096355573.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJW30_RS11920	2455730..2456617	-	888	WP_096355575.1	hydrogen peroxide-inducible genes activator	-
GJW30_RS11925	2456819..2458987	+	2169	WP_096355577.1	catalase/peroxidase HPI	-
GJW30_RS11930	2459230..2460411	-	1182	WP_157746744.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-82)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10571.08 Da
• Isoelectric Point: 9.8027

>T31034 WP_096355573.1 NZ_AP014946:c2455663-2455379 [Variibacter gotjawalensis]
VILNFKDRLTEAAFNGTAVKGFPAALLGVTRRKLRYLHAAHVLDDLRAPPGNRLEALTGDRKGQHSIRVNDQFRICFVWT
PNGPTEVEFVDYHA

Nucleotide

• Length: 285 bp

>T31034 NZ_AP014946:c2455663-2455379 [Variibacter gotjawalensis]
GTGATCCTGAATTTCAAAGATCGGCTGACCGAGGCGGCGTTCAATGGAACCGCTGTTAAGGGATTTCCGGCCGCCCTGCT
CGGTGTGACGCGGCGAAAGCTGAGATACTTGCATGCAGCCCATGTGCTGGACGACCTGCGCGCGCCGCCCGGCAACCGGC
TCGAAGCGCTGACGGGCGATCGCAAAGGTCAACACTCGATCCGCGTCAACGATCAATTTCGGATCTGCTTCGTCTGGACG
CCGAACGGGCCCACCGAGGTCGAATTCGTCGATTATCACGCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10931.69 Da
• Isoelectric Point: 8.0167

>AT31034 WP_096355571.1 NZ_AP014946:c2455358-2455056 [Variibacter gotjawalensis]
MAKTLPPMHPGEVLREEFLIPLNMSPGALARACDVPRTRIERLANEQTPVTADTALRLAKALGTSAELWLNLQADYDLQV
TRAKIGRDLKAIESVNPKAA

Nucleotide

• Length: 303 bp

>AT31034 NZ_AP014946:c2455358-2455056 [Variibacter gotjawalensis]
ATGGCCAAGACACTGCCGCCAATGCATCCCGGAGAGGTTCTCCGCGAAGAGTTTCTCATCCCACTCAATATGTCGCCGGG
CGCGCTCGCACGTGCCTGCGATGTACCGCGTACGCGGATCGAGCGATTGGCGAATGAGCAGACGCCCGTTACGGCCGATA
CGGCACTGCGTCTCGCGAAAGCGTTGGGTACGAGCGCCGAATTGTGGCTGAACCTGCAGGCGGACTACGATCTTCAAGTC
ACGCGCGCAAAGATCGGGCGCGATCTCAAGGCCATCGAGAGCGTCAATCCGAAGGCGGCATGA