Detailed information of TA system TA031004

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF3/-
• Location: 2026230..2026537
• Replicon: chromosome
• Accession: NZ_AP014921
• Organism: Staphylococcus aureus strain JH4899

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: JSA_RS10395
• Protein ID: WP_011447039.1
• Coordinates: 2026361..2026537 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	2026230..2026369 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSA_RS10335	2021796..2021975	+	180	WP_000669791.1	hypothetical protein	-
JSA_RS10345	2022286..2022546	+	261	WP_001791826.1	hypothetical protein	-
JSA_RS10350	2022599..2022949	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
JSA_RS10355	2023460..2023795	-	336	Protein_1912	SH3 domain-containing protein	-
JSA_RS10375	2024446..2024937	-	492	WP_000920038.1	staphylokinase	-
JSA_RS10380	2025128..2025883	-	756	WP_000861038.1	CHAP domain-containing protein	-
JSA_RS10385	2025895..2026149	-	255	WP_000611512.1	phage holin	-
JSA_RS10390	2026201..2026308	+	108	WP_031762631.1	hypothetical protein	-
-	2026230..2026369	+	140	NuclAT_0	-	Antitoxin
-	2026230..2026369	+	140	NuclAT_0	-	Antitoxin
-	2026230..2026369	+	140	NuclAT_0	-	Antitoxin
-	2026230..2026369	+	140	NuclAT_0	-	Antitoxin
JSA_RS10395	2026361..2026537	-	177	WP_011447039.1	putative holin-like toxin	Toxin
JSA_RS10400	2026740..2027513	-	774	WP_025174055.1	staphylococcal enterotoxin type P	-
JSA_RS10405	2027934..2028308	-	375	WP_000340977.1	hypothetical protein	-
JSA_RS10410	2028364..2028651	-	288	WP_001040255.1	hypothetical protein	-
JSA_RS10415	2028698..2028850	-	153	WP_001153681.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / sak / see / hlb / groEL	2022599..2078742	56143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T31004 WP_011447039.1 NZ_AP014921:c2026537-2026361 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T31004 NZ_AP014921:c2026537-2026361 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT31004 NZ_AP014921:2026230-2026369 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin