Detailed information of TA system TA030989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5218582..5219176
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: higB
• Uniprot ID: L8MR81
• Locus tag: KF707C_RS24230
• Protein ID: WP_003456758.1
• Coordinates: 5218898..5219176 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KF707C_RS24225
• Protein ID: WP_036994301.1
• Coordinates: 5218582..5218887 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS24195	5214230..5215171	+	942	WP_028629917.1	ribose-phosphate pyrophosphokinase	-
KF707C_RS24200	5215293..5215892	+	600	WP_003456751.1	50S ribosomal protein L25/general stress protein Ctc	-
KF707C_RS24205	5215957..5216541	+	585	WP_003456752.1	aminoacyl-tRNA hydrolase	-
KF707C_RS24210	5216570..5217670	+	1101	WP_003456754.1	redox-regulated ATPase YchF	-
KF707C_RS24220	5218249..5218539	-	291	WP_036994300.1	excalibur calcium-binding domain-containing protein	-
KF707C_RS24225	5218582..5218887	-	306	WP_036994301.1	HigA family addiction module antidote protein	Antitoxin
KF707C_RS24230	5218898..5219176	-	279	WP_003456758.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KF707C_RS24235	5219215..5220246	-	1032	WP_003456760.1	tyrosine-type recombinase/integrase	-
KF707C_RS24240	5220243..5221505	-	1263	WP_036994302.1	hypothetical protein	-
KF707C_RS24245	5221587..5222933	-	1347	WP_003456767.1	PulD	-
KF707C_RS24250	5222945..5224054	-	1110	WP_003456793.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5218249..5238382	20133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10770.38 Da
• Isoelectric Point: 8.5335

>T30989 WP_003456758.1 NZ_AP014862:c5219176-5218898 [Pseudomonas furukawaii]
MILSFKCADTRELFETGRSRRWSNILNAAIRKLVMLNAAVELRDLRSPPGNRLEALLHDRVGQYSIRINDQWRICFVWTD
AGPRDVEIVDYH

Nucleotide

• Length: 279 bp

>T30989 NZ_AP014862:c5219176-5218898 [Pseudomonas furukawaii]
ATGATCCTCAGCTTCAAGTGTGCAGATACGCGAGAGCTGTTTGAAACAGGCCGCTCGCGTCGCTGGAGCAACATCCTGAA
TGCCGCCATCCGCAAGCTGGTCATGCTCAACGCTGCGGTTGAACTACGAGATCTGCGATCGCCACCCGGCAACCGGCTGG
AAGCGCTTTTGCATGATCGGGTCGGTCAGTACAGCATCAGGATCAACGATCAATGGCGCATCTGCTTTGTCTGGACAGAC
GCCGGCCCCAGGGATGTAGAGATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11211.76 Da
• Isoelectric Point: 4.5158

>AT30989 WP_036994301.1 NZ_AP014862:c5218887-5218582 [Pseudomonas furukawaii]
MIVNGMRPVHPGEILREEFLEPLGIAPAALARALHVSAPTVNDIVRERRGVTADMALRLGRYFDTSAQFWMNLQSEYALA
TTYAESGEEIENEIEPLRAVG

Nucleotide

• Length: 306 bp

>AT30989 NZ_AP014862:c5218887-5218582 [Pseudomonas furukawaii]
ATGATTGTGAATGGAATGCGGCCCGTGCATCCGGGCGAGATCCTGCGTGAAGAGTTCCTGGAGCCATTGGGCATTGCTCC
AGCTGCGCTTGCTCGTGCGTTGCATGTATCCGCCCCGACGGTGAACGATATCGTCCGTGAGCGTCGTGGGGTAACGGCAG
ACATGGCCCTTCGGCTGGGCCGTTACTTCGACACGTCTGCCCAGTTCTGGATGAACCTGCAGAGTGAGTACGCTTTAGCG
ACCACTTACGCGGAGTCCGGCGAAGAAATCGAGAATGAGATCGAGCCGCTTCGTGCGGTTGGATAG

Structures

Toxin

• AlphaFold DB: L8MR81