Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4424576..4425270 Replicon chromosome
Accession NZ_AP014862
Organism Pseudomonas furukawaii strain KF707

Toxin (Protein)


Gene name higB Uniprot ID L8MTN5
Locus tag KF707C_RS20435 Protein ID WP_003456907.1
Coordinates 4424576..4424860 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KF707C_RS20440 Protein ID WP_003456906.1
Coordinates 4424869..4425270 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KF707C_RS20420 4419865..4420785 + 921 Protein_4005 LysR family transcriptional regulator -
KF707C_RS20425 4421122..4421490 - 369 WP_003456909.1 nitrite reductase small subunit NirD -
KF707C_RS20430 4421490..4424042 - 2553 WP_003456908.1 nitrite reductase large subunit NirB -
KF707C_RS20435 4424576..4424860 + 285 WP_003456907.1 type II toxin-antitoxin system HigB family toxin Toxin
KF707C_RS20440 4424869..4425270 + 402 WP_003456906.1 transcriptional regulator Antitoxin
KF707C_RS20445 4425274..4426113 - 840 WP_003456904.1 nitrilase family protein -
KF707C_RS20450 4426218..4427702 - 1485 WP_003456902.1 cytosine permease -
KF707C_RS20455 4427978..4428880 + 903 WP_003456900.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-90)

Antitoxin

Antitoxin HigA

(15-133)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11301.03 Da        Isoelectric Point: 10.1299

>T30987 WP_003456907.1 NZ_AP014862:4424576-4424860 [Pseudomonas furukawaii]
MHIITQKRIWEAKERWPEAASALDAWYRLMGRLEPTDFADMKQTFPSVDKVGDKHVFDIGGNKLRLIAVVDYRFRKVFIR
AVLDHREYDRNHWK

Download         Length: 285 bp

>T30987 NZ_AP014862:4424576-4424860 [Pseudomonas furukawaii]
ATGCACATAATCACTCAGAAGCGCATCTGGGAGGCGAAGGAGCGATGGCCTGAAGCCGCTTCGGCCCTGGACGCCTGGTA
TCGCCTGATGGGTCGCCTGGAGCCGACGGATTTCGCCGACATGAAGCAGACCTTCCCCAGCGTGGACAAGGTCGGCGACA
AGCATGTGTTCGATATCGGTGGCAACAAGCTCCGCCTGATCGCGGTCGTGGACTATCGCTTCAGAAAAGTCTTCATCCGT
GCCGTACTCGACCATCGCGAGTACGACCGCAACCATTGGAAGTGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14649.93 Da        Isoelectric Point: 5.1828

>AT30987 WP_003456906.1 NZ_AP014862:4424869-4425270 [Pseudomonas furukawaii]
MDDRLKLAKAHWHFVAPLLTPPRSEADYRTLVDALDELLDEIAGAPEHPLGGLAAQMGELIAAYDAVHFPMPKAPGREVL
RLLMQEHGLSQGDLPEVGAQSVVSEILAGKRQLNVRQIRALVERFQVPADLFI

Download         Length: 402 bp

>AT30987 NZ_AP014862:4424869-4425270 [Pseudomonas furukawaii]
ATGGACGACCGCTTGAAACTGGCCAAGGCACACTGGCACTTCGTTGCCCCGCTGCTCACCCCTCCCCGCTCCGAAGCGGA
CTACCGAACCCTGGTGGACGCCCTGGACGAGCTGCTGGACGAGATCGCGGGAGCACCGGAGCACCCGCTGGGCGGTCTGG
CCGCGCAGATGGGCGAGCTGATCGCCGCCTACGATGCGGTTCACTTCCCCATGCCCAAGGCCCCGGGACGAGAAGTGCTG
CGTCTGCTGATGCAGGAGCACGGACTGAGCCAGGGCGACCTGCCCGAGGTGGGCGCACAGTCCGTGGTCTCGGAAATCCT
GGCGGGCAAGCGTCAGCTGAACGTCCGCCAGATCCGTGCCCTGGTGGAGCGCTTCCAGGTACCCGCCGACCTCTTCATCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L8MTN5

Antitoxin

Source ID Structure

References