Detailed information of TA system TA030985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3548524..3549140
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: higB
• Uniprot ID: L8M7L1
• Locus tag: KF707C_RS16470
• Protein ID: WP_003448102.1
• Coordinates: 3548844..3549140 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KF707C_RS16465
• Protein ID: WP_003448104.1
• Coordinates: 3548524..3548835 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS16450	3544867..3547044	+	2178	WP_051050666.1	EAL domain-containing protein	-
KF707C_RS16455	3547570..3548040	-	471	WP_131679658.1	hypothetical protein	-
KF707C_RS16460	3548172..3548492	-	321	WP_036990781.1	transcriptional regulator	-
KF707C_RS16465	3548524..3548835	-	312	WP_003448104.1	HigA family addiction module antidote protein	Antitoxin
KF707C_RS16470	3548844..3549140	-	297	WP_003448102.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KF707C_RS16475	3549229..3549426	-	198	WP_003448100.1	hypothetical protein	-
KF707C_RS16480	3549588..3550175	+	588	WP_036990765.1	hypothetical protein	-
KF707C_RS16485	3550505..3551185	+	681	WP_131679657.1	hypothetical protein	-
KF707C_RS16490	3551780..3552052	-	273	WP_003448092.1	DUF971 domain-containing protein	-
KF707C_RS16495	3552049..3553014	-	966	WP_003448090.1	HEAT repeat domain-containing protein	-
KF707C_RS16500	3553011..3553868	-	858	WP_003448088.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11509.98 Da
• Isoelectric Point: 6.9894

>T30985 WP_003448102.1 NZ_AP014862:c3549140-3548844 [Pseudomonas furukawaii]
MIKSFRDTWLEAFFVHDEHAKQIPADISNRLFRKLQLLDDATTDSDLRIPPSNHFEKLVGKLKGLHSIRVNQQWRLVFEW
DGDKGEASGVYLDNHSYR

Nucleotide

• Length: 297 bp

>T30985 NZ_AP014862:c3549140-3548844 [Pseudomonas furukawaii]
ATGATCAAAAGCTTCAGGGACACATGGCTGGAAGCGTTCTTCGTTCATGACGAGCACGCCAAGCAGATACCGGCCGATAT
CAGCAACAGGCTCTTTCGAAAGCTGCAGTTGCTGGACGATGCCACTACCGACAGTGACTTGCGGATTCCACCTAGCAATC
ACTTCGAAAAACTGGTTGGCAAGTTGAAAGGGCTGCACTCCATACGGGTCAATCAGCAATGGCGATTGGTATTCGAGTGG
GATGGAGACAAAGGCGAAGCGTCGGGGGTGTACCTCGACAATCACTCTTACCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11718.49 Da
• Isoelectric Point: 8.0292

>AT30985 WP_003448104.1 NZ_AP014862:c3548835-3548524 [Pseudomonas furukawaii]
MIITERKPVSVGEMLTEEFLEPMGITQGQLAEAMGVQRKLVNELCNNRRAITADTALMLSRVFGNSADFWLNLQRRNDLW
EALNSPDRRARIERAKPLPHQAA

Nucleotide

• Length: 312 bp

>AT30985 NZ_AP014862:c3548835-3548524 [Pseudomonas furukawaii]
ATGATCATTACCGAACGTAAGCCGGTGAGCGTCGGCGAGATGCTCACCGAAGAGTTCCTGGAACCCATGGGCATTACTCA
GGGACAGTTGGCCGAAGCCATGGGCGTACAGCGCAAGCTGGTCAATGAGCTCTGCAACAACCGTCGCGCCATCACGGCGG
ACACTGCGTTGATGCTTTCCCGGGTCTTTGGTAACTCGGCCGATTTCTGGCTGAACCTGCAGCGCCGAAACGATCTCTGG
GAAGCGCTCAATTCGCCTGATCGCAGAGCCAGGATCGAGCGCGCTAAGCCACTTCCTCATCAGGCTGCTTGA

Structures

Toxin

• AlphaFold DB: L8M7L1