Detailed information of TA system TA030980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 537706..538364
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: higB
• Uniprot ID: L8MEK9
• Locus tag: KF707C_RS02440
• Protein ID: WP_003450776.1
• Coordinates: 538020..538364 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KF707C_RS02435
• Protein ID: WP_003450778.1
• Coordinates: 537706..538023 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS02415	533132..533902	-	771	WP_003450783.1	NERD domain-containing protein	-
KF707C_RS02420	534007..535146	-	1140	WP_003450782.1	Fic family protein	-
KF707C_RS02425	535675..536547	-	873	WP_036992394.1	AraC family transcriptional regulator	-
KF707C_RS02430	536687..537442	+	756	WP_003450780.1	SDR family oxidoreductase	-
KF707C_RS02435	537706..538023	-	318	WP_003450778.1	transcriptional regulator	Antitoxin
KF707C_RS02440	538020..538364	-	345	WP_003450776.1	hypothetical protein	Toxin
KF707C_RS02445	538412..538675	-	264	Protein_477	tyrosine-type recombinase/integrase	-
KF707C_RS02455	539058..542795	-	3738	WP_003450770.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	520222..557818	37596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13361.27 Da
• Isoelectric Point: 10.0218

>T30980 WP_003450776.1 NZ_AP014862:c538364-538020 [Pseudomonas furukawaii]
MEALFVELPPFERHRADYLDDEAFRTFQRALMKNPEAGDVIQGTGGLRKIRFADGRRQKGKRGGIRVIYYWWLGGAQFWL
FTLYGKDVQDDLNEQQKKRLKQLLQAEIDARTTP

Nucleotide

• Length: 345 bp

>T30980 NZ_AP014862:c538364-538020 [Pseudomonas furukawaii]
ATGGAAGCATTATTCGTTGAACTACCGCCCTTCGAGCGCCATCGAGCGGACTACCTGGATGACGAGGCCTTTCGCACCTT
CCAGCGCGCGCTGATGAAGAACCCGGAAGCCGGCGATGTGATCCAGGGAACCGGCGGCCTGCGAAAGATTCGCTTCGCCG
ACGGGCGACGACAGAAGGGCAAGCGCGGCGGCATCCGAGTCATCTACTACTGGTGGCTGGGTGGCGCGCAGTTCTGGCTG
TTCACCCTGTATGGGAAGGACGTCCAGGACGACCTGAACGAGCAGCAGAAGAAGCGACTCAAGCAGCTGCTGCAAGCTGA
AATTGATGCGAGGACGACACCGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11997.80 Da
• Isoelectric Point: 8.0361

>AT30980 WP_003450778.1 NZ_AP014862:c538023-537706 [Pseudomonas furukawaii]
VKRDIFAELVEGFEALADERQGKVTLRSHKVQLPELCEVSSEELVAIRQKLNLSRPVFAMYLRTNPRTLENWEQGRAKPN
AQATTLIRLVQKYPETIEHLAALGQ

Nucleotide

• Length: 318 bp

>AT30980 NZ_AP014862:c538023-537706 [Pseudomonas furukawaii]
GTGAAACGAGACATCTTTGCTGAGCTGGTTGAAGGCTTCGAGGCTTTGGCGGATGAACGCCAAGGCAAGGTGACTCTGCG
CTCGCACAAAGTCCAATTACCCGAACTGTGCGAAGTCAGCTCCGAGGAGCTGGTGGCGATCCGCCAGAAGCTGAACCTGT
CACGCCCGGTTTTCGCCATGTATCTGCGCACCAACCCGCGCACCTTGGAGAACTGGGAGCAAGGTCGGGCCAAGCCAAAC
GCCCAGGCGACTACGCTGATTCGTCTGGTGCAAAAGTATCCTGAAACTATCGAGCATCTAGCTGCTCTCGGTCAGTGA

Structures

Toxin

• AlphaFold DB: L8MEK9