Detailed information of TA system TA030972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3264434..3265233
• Replicon: chromosome
• Accession: NZ_AP014857
• Organism: Escherichia albertii strain EC06-170

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: AST80_RS15875
• Protein ID: WP_059271009.1
• Coordinates: 3264769..3265233 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: AST80_RS15870
• Protein ID: WP_060877482.1
• Coordinates: 3264434..3264769 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST80_RS15855	3260223..3260993	-	771	WP_059235438.1	2-dehydro-3-deoxyglucarate aldolase	-
AST80_RS15860	3261009..3262343	-	1335	WP_059241781.1	galactarate/glucarate/glycerate transporter GarP	-
AST80_RS15865	3262714..3264285	+	1572	WP_059235441.1	galactarate dehydratase	-
AST80_RS15870	3264434..3264769	+	336	WP_060877482.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AST80_RS15875	3264769..3265233	+	465	WP_059271009.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AST80_RS15880	3265288..3266097	-	810	WP_059235445.1	aga operon transcriptional regulator AgaR	-
AST80_RS15885	3266346..3267626	+	1281	WP_059235447.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AST80_RS15890	3267649..3268122	+	474	WP_059218168.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AST80_RS15895	3268133..3268912	+	780	WP_000406226.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
AST80_RS15900	3268902..3269780	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
AST80_RS15905	3269798..3270232	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17835.26 Da
• Isoelectric Point: 9.6804

>T30972 WP_059271009.1 NZ_AP014857:3264769-3265233 [Escherichia albertii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTQETEEKH

Nucleotide

• Length: 465 bp

>T30972 NZ_AP014857:3264769-3265233 [Escherichia albertii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAACCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACACTGCGCACCTACGGTAAAAAAACAGATGCCTATACAGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCAAGAGACAGAAGAAAAGCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12320.93 Da
• Isoelectric Point: 4.6672

>AT30972 WP_060877482.1 NZ_AP014857:3264434-3264769 [Escherichia albertii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQENHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT30972 NZ_AP014857:3264434-3264769 [Escherichia albertii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGAATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA