Detailed information of TA system TA030951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3586293..3587005
• Replicon: chromosome
• Accession: NZ_AP014856
• Organism: Escherichia albertii strain CB9786

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: AST72_RS18365
• Protein ID: WP_000162413.1
• Coordinates: 3586293..3586595 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AST72_RS18370
• Protein ID: WP_000806446.1
• Coordinates: 3586667..3587005 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST72_RS18350	3583156..3583998	+	843	WP_095576108.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-
AST72_RS18355	3584070..3585422	+	1353	WP_095576109.1	glutathione-disulfide reductase	-
AST72_RS18360	3585476..3585559	-	84	WP_001295215.1	hypothetical protein	-
AST72_RS23630	3585651..3586129	-	479	Protein_3416	hypothetical protein	-
AST72_RS18365	3586293..3586595	+	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AST72_RS18370	3586667..3587005	+	339	WP_000806446.1	HigA family addiction module antidote protein	Antitoxin
AST72_RS18375	3587062..3587241	+	180	WP_002460167.1	hypothetical protein	-
AST72_RS18380	3587688..3588986	+	1299	WP_095576110.1	pentapeptide repeat-containing protein	-
AST72_RS18385	3589029..3589595	+	567	WP_001044206.1	outer membrane lipoprotein Slp	-
AST72_RS18390	3589742..3590272	+	531	WP_095576111.1	LuxR family transcriptional regulator	-
AST72_RS18395	3590344..3591372	-	1029	WP_095576112.1	hematinate-forming heme oxygenase ChuS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T30951 WP_000162413.1 NZ_AP014856:3586293-3586595 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T30951 NZ_AP014856:3586293-3586595 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT30951 WP_000806446.1 NZ_AP014856:3586667-3587005 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT30951 NZ_AP014856:3586667-3587005 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7