Detailed information of TA system TA030936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256898..257592
• Replicon: chromosome
• Accession: NZ_AP014856
• Organism: Escherichia albertii strain CB9786

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AST72_RS01250
• Protein ID: WP_001263500.1
• Coordinates: 257194..257592 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AST72_RS01245
• Protein ID: WP_000554758.1
• Coordinates: 256898..257191 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST72_RS01225	252597..253094	+	498	WP_095573642.1	REP-associated tyrosine transposase RayT	-
AST72_RS01230	253210..254922	-	1713	Protein_228	flagellar biosynthesis protein FlhA	-
AST72_RS01235	254894..255661	+	768	WP_072243690.1	putative lateral flagellar export/assembly protein LafU	-
AST72_RS01240	255791..256846	+	1056	WP_059217800.1	DNA polymerase IV	-
AST72_RS01245	256898..257191	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AST72_RS01250	257194..257592	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AST72_RS01255	257602..258054	+	453	WP_095575504.1	GNAT family N-acetyltransferase	-
AST72_RS01260	258067..258666	+	600	WP_072249376.1	peptide chain release factor H	-
AST72_RS01270	258807..259049	-	243	WP_059222190.1	hypothetical protein	-
AST72_RS01275	259108..260565	-	1458	WP_001292996.1	cytosol nonspecific dipeptidase	-
AST72_RS01280	260826..261284	+	459	WP_001291989.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T30936 WP_001263500.1 NZ_AP014856:257194-257592 [Escherichia albertii]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T30936 NZ_AP014856:257194-257592 [Escherichia albertii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT30936 WP_000554758.1 NZ_AP014856:256898-257191 [Escherichia albertii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT30936 NZ_AP014856:256898-257191 [Escherichia albertii]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATATTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3