Detailed information of TA system TA030929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3521173..3521885
• Replicon: chromosome
• Accession: NZ_AP014855
• Organism: Escherichia albertii strain NIAH_Bird_3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: AST93_RS17500
• Protein ID: WP_000162413.1
• Coordinates: 3521173..3521475 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AST93_RS17505
• Protein ID: WP_000806446.1
• Coordinates: 3521547..3521885 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST93_RS17475	3517837..3518679	+	843	WP_000954227.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-
AST93_RS17480	3518751..3520103	+	1353	WP_054410173.1	glutathione-disulfide reductase	-
AST93_RS17485	3520157..3520240	-	84	WP_001295215.1	hypothetical protein	-
AST93_RS17490	3520268..3520441	+	174	WP_059235695.1	hypothetical protein	-
AST93_RS17495	3520515..3521009	-	495	WP_095574326.1	hypothetical protein	-
AST93_RS17500	3521173..3521475	+	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AST93_RS17505	3521547..3521885	+	339	WP_000806446.1	HigA family addiction module antidote protein	Antitoxin
AST93_RS17510	3521942..3522121	+	180	WP_002460167.1	hypothetical protein	-
AST93_RS17515	3522568..3523704	+	1137	WP_095574327.1	pentapeptide repeat-containing protein	-
AST93_RS17520	3523910..3524476	+	567	WP_001044206.1	outer membrane lipoprotein Slp	-
AST93_RS17525	3524623..3525153	+	531	WP_059220985.1	LuxR family transcriptional regulator	-
AST93_RS17530	3525225..3526253	-	1029	WP_001110409.1	hematinate-forming heme oxygenase ChuS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T30929 WP_000162413.1 NZ_AP014855:3521173-3521475 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T30929 NZ_AP014855:3521173-3521475 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT30929 WP_000806446.1 NZ_AP014855:3521547-3521885 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT30929 NZ_AP014855:3521547-3521885 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7