Detailed information of TA system TA030912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 287388..288082
• Replicon: chromosome
• Accession: NZ_AP014855
• Organism: Escherichia albertii strain NIAH_Bird_3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: AST93_RS01350
• Protein ID: WP_095573643.1
• Coordinates: 287684..288082 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AST93_RS01345
• Protein ID: WP_000554758.1
• Coordinates: 287388..287681 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST93_RS01325	283087..283584	+	498	WP_095573642.1	REP-associated tyrosine transposase RayT	-
AST93_RS01330	283700..285412	-	1713	Protein_249	flagellar biosynthesis protein FlhA	-
AST93_RS01335	285384..286151	+	768	WP_072243690.1	putative lateral flagellar export/assembly protein LafU	-
AST93_RS01340	286281..287336	+	1056	WP_059217800.1	DNA polymerase IV	-
AST93_RS01345	287388..287681	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AST93_RS01350	287684..288082	+	399	WP_095573643.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AST93_RS01355	288092..288544	+	453	WP_095573644.1	GNAT family N-acetyltransferase	-
AST93_RS01365	288863..290002	+	1140	WP_197702480.1	RNA ligase RtcB family protein	-
AST93_RS01370	289999..290613	+	615	WP_095573646.1	peptide chain release factor H	-
AST93_RS01375	290675..291184	+	510	WP_001361775.1	hydrolase	-
AST93_RS01385	291491..292948	-	1458	WP_095573647.1	cytosol nonspecific dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15441.90 Da
• Isoelectric Point: 8.9511

>T30912 WP_095573643.1 NZ_AP014855:287684-288082 [Escherichia albertii]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMVEAFRMRF

Nucleotide

• Length: 399 bp

>T30912 NZ_AP014855:287684-288082 [Escherichia albertii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGTAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT30912 WP_000554758.1 NZ_AP014855:287388-287681 [Escherichia albertii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT30912 NZ_AP014855:287388-287681 [Escherichia albertii]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3