Detailed information of TA system TA030904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4843947..4844628
• Replicon: chromosome
• Accession: NZ_AP014839
• Organism: Pseudomonas aeruginosa strain 8380

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PA8380_RS22465
• Protein ID: WP_015503432.1
• Coordinates: 4844263..4844628 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA8380_RS22460
• Protein ID: WP_016561576.1
• Coordinates: 4843947..4844270 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA8380_RS22435	4839839..4840522	-	684	WP_017148794.1	TetR/AcrR family transcriptional regulator	-
PA8380_RS22440	4840610..4841488	+	879	WP_003163191.1	hypothetical protein	-
PA8380_RS31480	4842091..4842318	+	228	WP_014603215.1	hypothetical protein	-
PA8380_RS31485	4842333..4842875	-	543	WP_019396018.1	tyrosine-type recombinase/integrase	-
PA8380_RS31490	4843050..4843325	-	276	WP_004353146.1	hypothetical protein	-
PA8380_RS22455	4843325..4843657	-	333	WP_016561578.1	hypothetical protein	-
PA8380_RS31920	4843712..4843831	+	120	Protein_4491	IS5/IS1182 family transposase	-
PA8380_RS22460	4843947..4844270	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
PA8380_RS22465	4844263..4844628	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA8380_RS22470	4844892..4845134	-	243	WP_043884955.1	hypothetical protein	-
PA8380_RS22475	4845341..4845613	+	273	WP_003085667.1	hypothetical protein	-
PA8380_RS22480	4845632..4846057	-	426	WP_003114206.1	ring-cleaving dioxygenase	-
PA8380_RS22485	4846158..4847042	+	885	WP_003454589.1	LysR family transcriptional regulator	-
PA8380_RS22490	4847015..4847968	-	954	WP_003085661.1	LysR family transcriptional regulator	-
PA8380_RS22495	4848189..4848623	+	435	WP_016253806.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T30904 WP_015503432.1 NZ_AP014839:c4844628-4844263 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T30904 NZ_AP014839:c4844628-4844263 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT30904 WP_016561576.1 NZ_AP014839:c4844270-4843947 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT30904 NZ_AP014839:c4844270-4843947 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA