Detailed information of TA system TA030876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6187740..6188412
• Replicon: chromosome
• Accession: NZ_AP014703
• Organism: Pseudomonas sp. St290

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PBDP_RS27185
• Protein ID: WP_109754987.1
• Coordinates: 6188047..6188412 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PBDP_RS27180
• Protein ID: WP_109754986.1
• Coordinates: 6187740..6188054 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PBDP_RS27170 (PBDP_5360)	6184787..6187219	+	2433	WP_249411826.1	hypothetical protein	-
PBDP_RS27175 (PBDP_5361)	6187256..6187717	+	462	WP_018607342.1	Lrp/AsnC family transcriptional regulator	-
PBDP_RS27180 (PBDP_5362)	6187740..6188054	-	315	WP_109754986.1	XRE family transcriptional regulator	Antitoxin
PBDP_RS27185 (PBDP_5363)	6188047..6188412	-	366	WP_109754987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PBDP_RS27190 (PBDP_5364)	6188773..6190455	+	1683	WP_212801543.1	NAD(P)/FAD-dependent oxidoreductase	-
PBDP_RS27195 (PBDP_5365)	6190468..6191262	+	795	WP_109754989.1	carbon-nitrogen hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14013.68 Da
• Isoelectric Point: 4.9669

>T30876 WP_109754987.1 NZ_AP014703:c6188412-6188047 [Pseudomonas sp. St290]
MKWDVEYTDEFEMWWSCLSETEQDSVQASVMLLGDAGPHLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGQDRWYQECVPLAERLYDEHLEALKREGFDNG

Nucleotide

• Length: 366 bp

>T30876 NZ_AP014703:c6188412-6188047 [Pseudomonas sp. St290]
ATGAAATGGGACGTTGAATACACCGACGAATTTGAGATGTGGTGGAGCTGTCTCAGTGAGACCGAGCAAGATTCAGTGCA
GGCCTCGGTGATGCTGCTTGGCGATGCCGGGCCGCATCTGGGTTTTCCTCACACCAGTGATATCAAGGGCTCGCGTCATG
GCAACCTTCGTGAGTTACGAGTACAACACGCAGGTAGGCCTTATCGTGTGCTCTATGCGTTTGATCCACGCCGTTGCGCC
ATCCTGCTGATCGGGGGAGACAAAACCGGCCAGGACCGTTGGTATCAGGAATGTGTGCCTTTGGCTGAACGTCTCTATGA
CGAGCATCTGGAAGCATTGAAGAGAGAGGGCTTTGACAATGGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12002.75 Da
• Isoelectric Point: 9.9863

>AT30876 WP_109754986.1 NZ_AP014703:c6188054-6187740 [Pseudomonas sp. St290]
MAKKFSDLIARRSPDSRARAETLYQKLRAEMPLHELRQAQELSQDALAKTLHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFAHQTQ

Nucleotide

• Length: 315 bp

>AT30876 NZ_AP014703:c6188054-6187740 [Pseudomonas sp. St290]
ATGGCTAAGAAATTTTCCGACTTGATTGCACGTCGTTCGCCTGACTCCCGGGCTCGTGCAGAGACTCTGTATCAGAAACT
GCGTGCAGAAATGCCGTTGCACGAGTTGCGTCAGGCGCAGGAGCTGAGCCAGGATGCTTTGGCCAAGACGCTGCATATCA
ACCAGGCGGCAGTCTCGAAAATGGAACGCCGTACCGACATGTACATCAGTACGCTACGAAATTACATCCGCGCCATGGGG
GGGGAATTGGAGATTATCGCGACCTTCCCGGAGGGGCAGGTCAGGATCGAAAACTTCGCCCATCAAACACAGTAA