Detailed information of TA system TA030874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2216101..2216705
• Replicon: chromosome
• Accession: NZ_AP014703
• Organism: Pseudomonas sp. St290

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PBDP_RS09725
• Protein ID: WP_116831887.1
• Coordinates: 2216391..2216705 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6N1CCA5
• Locus tag: PBDP_RS09720
• Protein ID: WP_058545613.1
• Coordinates: 2216101..2216388 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PBDP_RS09695 (PBDP_1918)	2212158..2213207	+	1050	WP_212802487.1	NAD(P)-dependent alcohol dehydrogenase	-
PBDP_RS09700 (PBDP_1919)	2213334..2214551	+	1218	WP_212802153.1	MFS transporter	-
PBDP_RS09705 (PBDP_1920)	2214563..2214970	-	408	WP_163854612.1	DoxX family protein	-
PBDP_RS09710 (PBDP_1921)	2215028..2215456	+	429	WP_240337723.1	helix-turn-helix domain-containing protein	-
PBDP_RS09715 (PBDP_1922)	2215472..2215957	-	486	WP_212802154.1	hypothetical protein	-
PBDP_RS09720 (PBDP_1923)	2216101..2216388	-	288	WP_058545613.1	helix-turn-helix domain-containing protein	Antitoxin
PBDP_RS09725 (PBDP_1924)	2216391..2216705	-	315	WP_116831887.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PBDP_RS09730 (PBDP_1925)	2216836..2217267	+	432	WP_212802155.1	arsenic resistance N-acetyltransferase ArsN2	-
PBDP_RS09735 (PBDP_1926)	2217264..2218622	-	1359	WP_212802156.1	chromate efflux transporter	-
PBDP_RS09740 (PBDP_1927)	2218801..2219460	+	660	WP_116831889.1	ribonuclease T2	-
PBDP_RS09745 (PBDP_1928)	2219672..2221243	+	1572	WP_212802157.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11934.03 Da
• Isoelectric Point: 10.1359

>T30874 WP_116831887.1 NZ_AP014703:c2216705-2216391 [Pseudomonas sp. St290]
MIFIETPIFTRRVKELMDDDDFAVLQKILVCNPSAGDVMEATGGIRKIRIAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKALKLIVEHWR

Nucleotide

• Length: 315 bp

>T30874 NZ_AP014703:c2216705-2216391 [Pseudomonas sp. St290]
ATGATTTTTATTGAAACTCCAATTTTTACCCGTCGGGTCAAAGAACTGATGGATGACGATGATTTCGCTGTCCTACAGAA
AATACTGGTCTGCAATCCTTCCGCTGGTGATGTCATGGAAGCCACCGGAGGGATCCGAAAGATTCGTATCGCAGCCAAAG
GCCATGGCAAACGGGGTGGGGCCAGGGTGATCTACTACCACTTCGTCCATGCTTCGCGGATCGCATTACTGATGATATAT
CCGAAAAATGAACAGCAAGACTTGACCGTCGATCAACGCAAAGCATTGAAATTGATTGTCGAGCACTGGAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.40 Da
• Isoelectric Point: 8.7885

>AT30874 WP_058545613.1 NZ_AP014703:c2216388-2216101 [Pseudomonas sp. St290]
MSKFFDELMESVQQMDEIVRGERQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVISALAV

Nucleotide

• Length: 288 bp

>AT30874 NZ_AP014703:c2216388-2216101 [Pseudomonas sp. St290]
ATGAGCAAATTTTTCGATGAGCTGATGGAAAGCGTACAACAGATGGATGAAATCGTCCGAGGCGAACGCCAGCCGTCACG
GGAGTTCATCGTCGATGCGTTGCAAGTGAAGGAAATACGCAAGGCTACCGGCCTGACCCAAGCCAAGTTCGCAGCACTGA
TCGATGTCCAACTGGGCACGCTTCGCAACTGGGAGCAGGGACGACGCGAGCCTACCGGCCCCGCTAAAGCACTGCTGCGG
GCGATTCATAATGATCCGAAACACGTGATCAGCGCCCTAGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6N1CCA5