Detailed information of TA system TA030870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4053205..4053808
• Replicon: plasmid pLOOC260-2
• Accession: NZ_AP014685
• Organism: Bradyrhizobium diazoefficiens strain NK6

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: NK6_RS51280
• Protein ID: WP_082755755.1
• Coordinates: 4053205..4053492 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: NK6_RS19745
• Protein ID: WP_060909739.1
• Coordinates: 4053509..4053808 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NK6_RS19715	4050128..4050412	-	285	WP_060909737.1	IS66 family insertion sequence element accessory protein TnpB	-
NK6_RS19720	4050409..4050807	-	399	WP_026192391.1	IS66 family insertion sequence hypothetical protein	-
NK6_RS51275	4051331..4051525	-	195	WP_080586571.1	hypothetical protein	-
NK6_RS19730	4052057..4052308	-	252	WP_060909738.1	hypothetical protein	-
NK6_RS19735	4052308..4052589	-	282	WP_038943178.1	hypothetical protein	-
NK6_RS19740	4052826..4053029	+	204	WP_049807601.1	hypothetical protein	-
NK6_RS51280	4053205..4053492	+	288	WP_082755755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NK6_RS19745	4053509..4053808	+	300	WP_060909739.1	HigA family addiction module antidote protein	Antitoxin
NK6_RS56570	4053805..4053948	-	144	WP_171901171.1	hypothetical protein	-
NK6_RS19750	4053993..4054583	+	591	WP_060909740.1	hypothetical protein	-
NK6_RS19755	4054757..4054945	+	189	WP_060909741.1	DUF2158 domain-containing protein	-
NK6_RS19760	4054974..4055516	-	543	WP_060909742.1	hypothetical protein	-
NK6_RS19765	4055675..4057018	+	1344	WP_039183842.1	IS1380-like element ISBdi2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tufA / gmd / gmd / kdsB / htpB / acpXL / adeG / adeG / acpXL / kdsA	1..9780023	9780023
	-	flank	IS/Tn	-	-	4050128..4050412	284
	-	flank	IS/Tn	-	-	4055675..4057018	1343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11104.75 Da
• Isoelectric Point: 9.6539

>T30870 WP_082755755.1 NZ_AP014685:4053205-4053492 [Bradyrhizobium diazoefficiens]
MAIRSFKDANTKAVFDGNCPRGFPTTIVRVARRKVRMVDAAKELFDLKAPPNNKLHELEKDRKGQHAIWINKQFRVCFVW
RDGEAYDVEIVDYHD

Nucleotide

• Length: 288 bp

>T30870 NZ_AP014685:4053205-4053492 [Bradyrhizobium diazoefficiens]
ATGGCCATTCGGTCTTTCAAGGACGCCAACACCAAGGCGGTTTTTGACGGCAACTGCCCCCGCGGGTTCCCGACAACAAT
TGTCAGGGTGGCTCGTCGGAAAGTCCGAATGGTTGACGCTGCAAAGGAGCTATTCGATCTGAAAGCACCGCCAAACAACA
AGCTTCATGAACTGGAAAAAGATCGCAAAGGACAGCACGCGATTTGGATCAACAAACAGTTCCGAGTTTGCTTTGTTTGG
AGAGATGGCGAAGCGTATGACGTTGAGATCGTAGATTATCACGACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11220.06 Da
• Isoelectric Point: 7.1674

>AT30870 WP_060909739.1 NZ_AP014685:4053509-4053808 [Bradyrhizobium diazoefficiens]
MAKLKPLHPGEVLREEFMEPYGLTPYALANLCHIPRTRIERIAREETGITADTALRLAKVFGTTPDFWLTLQSRFEVEKT
GAEIASELKQIKPRELVVA

Nucleotide

• Length: 300 bp

>AT30870 NZ_AP014685:4053509-4053808 [Bradyrhizobium diazoefficiens]
ATGGCAAAGTTGAAGCCACTGCACCCGGGAGAGGTCCTGCGGGAAGAATTTATGGAGCCCTACGGCCTAACTCCGTACGC
CCTTGCGAACCTGTGCCATATCCCGCGCACTCGCATTGAGCGCATTGCCCGGGAGGAAACGGGTATTACGGCTGATACCG
CATTGCGTCTCGCAAAGGTATTCGGCACCACGCCCGACTTCTGGCTTACGCTGCAATCGAGGTTTGAGGTCGAAAAGACC
GGCGCCGAGATTGCTAGCGAGCTGAAGCAGATCAAACCACGTGAATTGGTAGTGGCCTAA