Detailed information of TA system TA030858

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 1999461..1999768
• Replicon: chromosome
• Accession: NZ_AP014653
• Organism: Staphylococcus aureus strain TMUS2134

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: SAJPND4_RS14445
• Protein ID: WP_011447039.1
• Coordinates: 1999592..1999768 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	1999461..1999600 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAJPND4_RS09940	1994812..1995072	+	261	WP_001791826.1	hypothetical protein	-
SAJPND4_RS09945	1995125..1995465	-	341	Protein_1872	complement inhibitor SCIN-A	-
SAJPND4_RS09950	1996148..1996597	+	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
SAJPND4_RS14790	1996692..1997027	-	336	Protein_1874	SH3 domain-containing protein	-
SAJPND4_RS09960	1997677..1998168	-	492	WP_000919350.1	staphylokinase	-
SAJPND4_RS09965	1998359..1999114	-	756	WP_000861038.1	CHAP domain-containing protein	-
SAJPND4_RS09970	1999126..1999380	-	255	WP_000611512.1	phage holin	-
SAJPND4_RS09975	1999432..1999539	+	108	WP_001791821.1	hypothetical protein	-
-	1999461..1999600	+	140	NuclAT_0	-	Antitoxin
-	1999461..1999600	+	140	NuclAT_0	-	Antitoxin
-	1999461..1999600	+	140	NuclAT_0	-	Antitoxin
-	1999461..1999600	+	140	NuclAT_0	-	Antitoxin
SAJPND4_RS14445	1999592..1999768	-	177	WP_011447039.1	putative holin-like toxin	Toxin
SAJPND4_RS09980	1999918..2000214	-	297	WP_000539688.1	DUF2951 domain-containing protein	-
SAJPND4_RS09985	2000272..2000559	-	288	WP_001040261.1	hypothetical protein	-
SAJPND4_RS09990	2000606..2000758	-	153	WP_001153681.1	hypothetical protein	-
SAJPND4_RS09995	2000748..2004533	-	3786	WP_000582165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / scn / chp / sak / hlb / groEL	1995125..2047458	52333

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T30858 WP_011447039.1 NZ_AP014653:c1999768-1999592 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T30858 NZ_AP014653:c1999768-1999592 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT30858 NZ_AP014653:1999461-1999600 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin