Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5953054..5953649 Replicon chromosome
Accession NZ_AP014651
Organism Pseudomonas aeruginosa strain NCGM257

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag PA257_RS34740 Protein ID WP_003113526.1
Coordinates 5953371..5953649 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag PA257_RS28145 Protein ID WP_003111575.1
Coordinates 5953054..5953359 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA257_RS34730 5948842..5949516 - 675 WP_079759003.1 DUF2290 domain-containing protein -
PA257_RS34735 5949513..5951720 - 2208 WP_079381482.1 DEAD/DEAH box helicase -
PA257_RS35260 5952127..5952717 - 591 WP_108703096.1 ankyrin repeat domain-containing protein -
PA257_RS28145 5953054..5953359 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
PA257_RS34740 5953371..5953649 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PA257_RS28150 5953978..5956206 + 2229 WP_033991161.1 TonB-dependent receptor -
PA257_RS28155 5956277..5956924 - 648 WP_003095021.1 carbonate dehydratase -
PA257_RS28160 5956986..5958224 - 1239 WP_031686604.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T30838 WP_003113526.1 NZ_AP014651:c5953649-5953371 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T30838 NZ_AP014651:c5953649-5953371 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGACGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT30838 WP_003111575.1 NZ_AP014651:c5953359-5953054 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT30838 NZ_AP014651:c5953359-5953054 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References