Detailed information of TA system TA030808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1547466..1548070
• Replicon: chromosome
• Accession: NZ_AP014637
• Organism: Pseudomonas sp. StFLB209

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A077LCM0
• Locus tag: PSCI_RS07175
• Protein ID: WP_045484686.1
• Coordinates: 1547466..1547780 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PSCI_RS07180
• Protein ID: WP_045484689.1
• Coordinates: 1547783..1548070 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSCI_RS28455	1543265..1543438	-	174	WP_084709882.1	DUF1427 family protein	-
PSCI_RS07150	1543656..1544426	-	771	WP_045484673.1	helix-turn-helix transcriptional regulator	-
PSCI_RS07155	1544630..1545256	+	627	WP_045484675.1	hydrolase	-
PSCI_RS07160	1545396..1545977	+	582	WP_045484677.1	TIGR00730 family Rossman fold protein	-
PSCI_RS07165	1545985..1546653	-	669	WP_045484680.1	haloacid dehalogenase type II	-
PSCI_RS07170	1546682..1547119	-	438	WP_045484683.1	hypothetical protein	-
PSCI_RS07175	1547466..1547780	+	315	WP_045484686.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSCI_RS07180	1547783..1548070	+	288	WP_045484689.1	helix-turn-helix domain-containing protein	Antitoxin
PSCI_RS07185	1548295..1548795	-	501	WP_045484692.1	GNAT family N-acetyltransferase	-
PSCI_RS07190	1549046..1550590	-	1545	WP_045484695.1	phosphoenolpyruvate carboxykinase	-
PSCI_RS07195	1550772..1551677	-	906	WP_045484698.1	Hsp33 family molecular chaperone HslO	-
PSCI_RS07200	1551751..1552155	-	405	WP_045484701.1	RNA-binding protein S4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-71)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11655.55 Da
• Isoelectric Point: 10.3138

>T30808 WP_045484686.1 NZ_AP014637:1547466-1547780 [Pseudomonas sp. StFLB209]
MIFIETPVFTKRAKELLEDAEYGDLLKDLLKNPSKGVLIEGTGGIRKIRVASRGRGTRGGARAIYYHFVSASRIAMLMIY
AKNEKHDLSSDERKALKTIIDGWS

Nucleotide

• Length: 315 bp

>T30808 NZ_AP014637:1547466-1547780 [Pseudomonas sp. StFLB209]
ATGATTTTTATCGAGACACCTGTATTTACAAAGCGCGCTAAAGAACTGCTTGAAGACGCAGAGTACGGTGATCTACTGAA
GGACCTCCTAAAAAACCCCTCAAAGGGCGTGTTGATTGAGGGAACCGGGGGTATCCGAAAGATTCGCGTCGCCTCAAGAG
GTCGCGGCACACGTGGCGGTGCAAGGGCTATCTACTATCACTTCGTTTCAGCGTCCAGGATCGCCATGCTTATGATCTAT
GCGAAGAATGAAAAACACGATCTGAGCTCGGACGAACGAAAAGCATTGAAAACCATCATCGACGGATGGAGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10554.13 Da
• Isoelectric Point: 10.0863

>AT30808 WP_045484689.1 NZ_AP014637:1547783-1548070 [Pseudomonas sp. StFLB209]
MSKFFDELMESVQQMDGIIRGEKAASREFTVDALQVKEIRRSTGLSQAKFAAMIDVQIGTLRNWEQGRREPAGPARALLK
AIHNDPQNVLKALAG

Nucleotide

• Length: 288 bp

>AT30808 NZ_AP014637:1547783-1548070 [Pseudomonas sp. StFLB209]
ATGAGCAAATTTTTCGATGAATTAATGGAAAGCGTCCAGCAAATGGATGGGATCATTCGTGGAGAAAAAGCAGCGTCGCG
CGAGTTCACGGTGGACGCACTGCAGGTCAAAGAGATACGCCGCTCAACCGGCCTGTCTCAAGCGAAATTCGCTGCAATGA
TCGATGTGCAGATAGGCACGTTGCGTAACTGGGAACAGGGCCGTCGTGAGCCCGCAGGACCTGCACGTGCACTACTGAAA
GCTATTCACAACGATCCCCAGAACGTTCTCAAGGCCCTTGCTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A077LCM0