Detailed information of TA system TA030798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2677042..2677627
• Replicon: chromosome
• Accession: NZ_AP014630
• Organism: Acinetobacter guillouiae strain NBRC 110550

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A077KZ82
• Locus tag: AS4_RS12390
• Protein ID: WP_034617593.1
• Coordinates: 2677307..2677627 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AS4_RS12385
• Protein ID: WP_096734348.1
• Coordinates: 2677042..2677314 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS4_RS12370	2672382..2673002	-	621	WP_004719624.1	2OG-Fe(II) oxygenase	-
AS4_RS12375	2673193..2675355	-	2163	WP_096734347.1	malate synthase G	-
AS4_RS12380	2675953..2676546	-	594	WP_004719619.1	nitroreductase family protein	-
AS4_RS12385	2677042..2677314	-	273	WP_096734348.1	helix-turn-helix domain-containing protein	Antitoxin
AS4_RS12390	2677307..2677627	-	321	WP_034617593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AS4_RS12395	2677754..2678860	-	1107	WP_096734349.1	signal recognition particle-docking protein FtsY	-
AS4_RS12400	2679037..2680431	+	1395	WP_096734350.1	insulinase family protein	-
AS4_RS12405	2680445..2682055	+	1611	WP_096734351.1	insulinase family protein	-
AS4_RS12410	2682066..2682392	-	327	WP_096734352.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (35-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12139.95 Da
• Isoelectric Point: 8.0125

>T30798 WP_034617593.1 NZ_AP014630:c2677627-2677307 [Acinetobacter guillouiae]
MLFIETSIFTKQIKELVTDDEYRQLQQNLLVQPDKGDIVKNGGGIRKVRCAQGDKGKSGGIRVIYYWVSEDDHIFFLVAY
PKSVKDNLTDKETAILRQLVKEQLHG

Nucleotide

• Length: 321 bp

>T30798 NZ_AP014630:c2677627-2677307 [Acinetobacter guillouiae]
ATGTTATTCATTGAAACTAGTATTTTTACTAAACAAATCAAAGAGCTTGTGACGGATGATGAATATCGTCAGCTTCAACA
AAATCTATTGGTACAACCTGATAAGGGTGATATTGTTAAAAATGGCGGTGGTATCCGTAAAGTACGCTGTGCTCAAGGTG
ATAAAGGAAAAAGTGGTGGAATCCGCGTGATCTATTATTGGGTTTCAGAGGATGATCATATTTTCTTTTTAGTCGCGTAC
CCTAAGTCTGTAAAAGATAATTTAACGGATAAAGAAACAGCAATTCTGCGCCAATTAGTGAAGGAGCAACTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10209.57 Da
• Isoelectric Point: 6.4682

>AT30798 WP_096734348.1 NZ_AP014630:c2677314-2677042 [Acinetobacter guillouiae]
MDNNLFDDLVSSIKEAGEIKRQEIQASRVTELELPDIKAVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDTHPSLI

Nucleotide

• Length: 273 bp

>AT30798 NZ_AP014630:c2677314-2677042 [Acinetobacter guillouiae]
ATGGATAACAATTTATTTGATGACCTTGTTTCTTCGATTAAAGAAGCAGGAGAGATTAAACGTCAAGAGATTCAAGCAAG
TCGAGTCACTGAGCTTGAATTACCTGATATTAAAGCAGTACGTGAGAAAACAGGTTTAAGCCAAAATGAATTTGCTGCAC
GCTTGCATATTAGTCCTCGCACGCTTCAAAACTGGGAGCAAGGTAGACGTTATCCGACTGGTCCAGCTGCGACTTTAATC
CGTATTCTAGATACGCATCCAAGCCTAATTTAA

Structures

Toxin

• AlphaFold DB: A0A077KZ82