Detailed information of TA system TA030775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2124890..2125483
• Replicon: chromosome
• Accession: NZ_AP014623
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PCAU_RS09860
• Protein ID: WP_096373593.1
• Coordinates: 2125205..2125483 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: PCAU_RS09855
• Protein ID: WP_096373592.1
• Coordinates: 2124890..2125195 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCAU_RS09830	2120212..2120523	+	312	WP_096373588.1	hypothetical protein	-
PCAU_RS09835	2120551..2120724	+	174	WP_172900845.1	hypothetical protein	-
PCAU_RS09840	2120885..2121736	+	852	WP_096373589.1	hypothetical protein	-
PCAU_RS31975	2122557..2123198	-	642	WP_157756450.1	hypothetical protein	-
PCAU_RS09850	2123507..2124736	-	1230	WP_096373591.1	hypothetical protein	-
PCAU_RS09855	2124890..2125195	-	306	WP_096373592.1	HigA family addiction module antidote protein	Antitoxin
PCAU_RS09860	2125205..2125483	-	279	WP_096373593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PCAU_RS09865	2125609..2125890	-	282	WP_157756452.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
PCAU_RS31980	2126240..2127133	+	894	WP_157756453.1	hypothetical protein	-
PCAU_RS09870	2127358..2127723	-	366	WP_096373595.1	Arc family DNA-binding protein	-
PCAU_RS32235	2127863..2128009	+	147	WP_172900835.1	hypothetical protein	-
PCAU_RS09875	2128077..2128385	+	309	WP_096373596.1	DUF3077 domain-containing protein	-
PCAU_RS09880	2128385..2129176	+	792	WP_096373597.1	phage antirepressor KilAC domain-containing protein	-
PCAU_RS09885	2129598..2130143	+	546	WP_096373598.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2080728..2172972	92244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10342.78 Da
• Isoelectric Point: 8.5136

>T30775 WP_096373593.1 NZ_AP014623:c2125483-2125205 [Pseudomonas chlororaphis subsp. aurantiaca]
MIISFGCDETESLYRTGSSRRWVGILSVAQRKLTMLDAATSIQDLKSPPGNRLEALQGNRKGQHSIRINDQFRICFIWGA
YGPESVAIVDYH

Nucleotide

• Length: 279 bp

>T30775 NZ_AP014623:c2125483-2125205 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGATCATAAGCTTTGGCTGCGACGAGACAGAAAGTCTCTACAGAACAGGGTCCTCCCGGAGATGGGTGGGAATCCTTTC
GGTCGCACAAAGAAAACTGACCATGCTCGATGCAGCAACAAGCATTCAGGATCTGAAATCGCCGCCTGGCAATCGGCTGG
AAGCGCTACAAGGGAACAGAAAGGGTCAACACAGCATCCGAATTAATGACCAGTTCAGAATTTGCTTCATCTGGGGTGCG
TATGGGCCAGAAAGCGTTGCGATTGTAGACTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11138.67 Da
• Isoelectric Point: 6.2108

>AT30775 WP_096373592.1 NZ_AP014623:c2125195-2124890 [Pseudomonas chlororaphis subsp. aurantiaca]
VFTNGMRPVHPGEILREEYLHPMDLSPAALAKLLNVSAPTVNDIVRGRRNISADVALRLAACFDTSPQFWANLQSAYDLR
KAEIDHGAEIAQQVQRAPECA

Nucleotide

• Length: 306 bp

>AT30775 NZ_AP014623:c2125195-2124890 [Pseudomonas chlororaphis subsp. aurantiaca]
GTGTTTACTAACGGTATGCGCCCTGTACATCCGGGCGAAATTCTCAGGGAAGAGTATCTCCATCCCATGGATCTTTCTCC
AGCAGCTCTTGCGAAGCTGTTGAATGTGTCTGCACCTACAGTCAACGACATTGTACGTGGCCGTCGCAATATCAGTGCAG
ACGTAGCTCTACGCTTGGCTGCTTGTTTTGATACGAGCCCACAGTTCTGGGCGAATCTTCAGTCCGCCTATGACCTGCGG
AAAGCAGAAATTGACCATGGCGCTGAAATTGCTCAGCAGGTTCAACGCGCACCAGAATGTGCCTAA