Detailed information of TA system TA030773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 357906..358600
• Replicon: chromosome
• Accession: NZ_AP014623
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PCAU_RS01625
• Protein ID: WP_053268076.1
• Coordinates: 357906..358202 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A554PLA7
• Locus tag: PCAU_RS01630
• Protein ID: WP_009046631.1
• Coordinates: 358199..358600 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCAU_RS01590	353441..353986	-	546	WP_009046626.1	membrane protein	-
PCAU_RS01595	353983..354237	-	255	WP_007926835.1	acyl carrier protein	-
PCAU_RS01600	354247..354507	-	261	WP_007926834.1	acyl carrier protein	-
PCAU_RS01605	354482..355297	-	816	WP_096372882.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
PCAU_RS01610	355273..356004	-	732	WP_096372884.1	beta-ketoacyl synthase chain length factor	-
PCAU_RS01615	356337..357107	+	771	WP_007926828.1	ParA family protein	-
PCAU_RS01620	357234..357668	-	435	WP_007926827.1	thioredoxin TrxC	-
PCAU_RS01625	357906..358202	+	297	WP_053268076.1	type II toxin-antitoxin system HigB family toxin	Toxin
PCAU_RS01630	358199..358600	+	402	WP_009046631.1	transcriptional regulator	Antitoxin
PCAU_RS01635	358604..360067	-	1464	WP_096372885.1	YdiU family protein	-
PCAU_RS01640	360116..363466	-	3351	WP_096372886.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Antitoxin HigA (15-133)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11588.41 Da
• Isoelectric Point: 10.6515

>T30773 WP_053268076.1 NZ_AP014623:357906-358202 [Pseudomonas chlororaphis subsp. aurantiaca]
MHIITAKRIWEAKSRWPQSANALETWYRKVKSLNPADFAAIKAVFPATDKVGAFHVFDIGGNKLRIIAVVRYEVRRLYIR
HVLDHHEYDKGRWKEETR

Nucleotide

• Length: 297 bp

>T30773 NZ_AP014623:357906-358202 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCATATCATCACCGCCAAACGAATCTGGGAGGCCAAGAGCCGCTGGCCACAATCGGCCAATGCGCTGGAGACCTGGTA
CAGAAAAGTCAAAAGCCTCAATCCGGCCGATTTTGCAGCCATCAAGGCAGTATTTCCGGCCACCGACAAAGTGGGGGCGT
TTCACGTCTTCGATATTGGCGGCAACAAGTTACGAATCATCGCCGTGGTGCGTTATGAGGTGCGACGTCTCTATATCAGA
CACGTGCTGGACCATCACGAATACGACAAGGGCAGGTGGAAGGAGGAGACCAGATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14840.85 Da
• Isoelectric Point: 4.4394

>AT30773 WP_009046631.1 NZ_AP014623:358199-358600 [Pseudomonas chlororaphis subsp. aurantiaca]
MNVLVKQAADHWHFVAPVLRKPNTEADYDALVAALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPIASGVDVL
RSLMREHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQIRWLAERFGVSAEVFI

Nucleotide

• Length: 402 bp

>AT30773 NZ_AP014623:358199-358600 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAACGTTCTGGTGAAGCAGGCGGCGGATCATTGGCATTTCGTGGCCCCTGTGTTGCGTAAACCCAATACTGAAGCCGA
TTACGATGCATTGGTCGCGGCGCTGGATGAACTGCTGGATCTTATGGGAGAGGACGAGAACCATCCAGTGACTTCGCTGG
TGGACATCATCGGCGATTGGATCGAAGCCTACGACGAAGAGCATCGACCGATGCCGATAGCCAGTGGCGTTGACGTCTTG
CGCTCCCTGATGCGCGAACACGGCCTGACCCAGAGTGATCTGCCAGGCGTGGGTGCGCAGTCGGTGGTTTCGGAAGTACT
GAGCGGCAAGCGCCAGCTCAACCTGCGACAGATCCGCTGGCTGGCCGAGCGCTTCGGTGTTTCGGCCGAGGTATTTATCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A554PLA7