Detailed information of TA system TA030762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2797699..2798312
• Replicon: chromosome
• Accession: NZ_AP014582
• Organism: Bordetella bronchiseptica strain S798

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: BBS798_RS13070
• Protein ID: WP_003811545.1
• Coordinates: 2798001..2798312 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBS798_RS13065
• Protein ID: WP_003811547.1
• Coordinates: 2797699..2797998 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBS798_RS13055	2793807..2794481	-	675	WP_003811550.1	response regulator transcription factor	-
BBS798_RS13060	2794611..2797685	+	3075	WP_033455244.1	autotransporter SphB2	-
BBS798_RS13065	2797699..2797998	-	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
BBS798_RS13070	2798001..2798312	-	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBS798_RS13075	2798426..2799049	-	624	WP_003811542.1	glutathione S-transferase family protein	-
BBS798_RS13080	2799168..2800121	-	954	WP_033451213.1	LysR family transcriptional regulator	-
BBS798_RS13085	2800239..2801201	+	963	WP_003811538.1	tripartite tricarboxylate transporter substrate binding protein	-
BBS798_RS13090	2801216..2802610	+	1395	WP_003811536.1	arylsulfatase	-
BBS798_RS13095	2802607..2803194	+	588	WP_003811534.1	LamB/YcsF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T30762 WP_003811545.1 NZ_AP014582:c2798312-2798001 [Bordetella bronchiseptica]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T30762 NZ_AP014582:c2798312-2798001 [Bordetella bronchiseptica]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAGCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT30762 WP_003811547.1 NZ_AP014582:c2797998-2797699 [Bordetella bronchiseptica]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT30762 NZ_AP014582:c2797998-2797699 [Bordetella bronchiseptica]
ATGAAAAAAGACAACACGGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3