Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2980985..2981633 Replicon chromosome
Accession NZ_AP014573
Organism Mycobacterium tuberculosis str. Kurono

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag KURONO_RS14075 Protein ID WP_003899414.1
Coordinates 2980985..2981308 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag KURONO_RS14080 Protein ID WP_003899415.1
Coordinates 2981388..2981633 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KURONO_RS14055 2976559..2977820 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
KURONO_RS22260 2977847..2978032 - 186 WP_009935348.1 hypothetical protein -
KURONO_RS14060 2978194..2979633 - 1440 WP_003899411.1 phage major capsid protein -
KURONO_RS14065 2979641..2980174 - 534 WP_003899412.1 HK97 family phage prohead protease -
KURONO_RS21635 2980327..2980818 - 492 WP_003900541.1 phage terminase small subunit P27 family -
KURONO_RS14075 2980985..2981308 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
KURONO_RS14080 2981388..2981633 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
KURONO_RS14085 2981630..2983057 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
KURONO_RS14090 2983059..2983451 - 393 WP_003899417.1 DUF2742 domain-containing protein -
KURONO_RS14095 2983448..2983708 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
KURONO_RS14100 2983725..2984087 - 363 WP_003900543.1 hypothetical protein -
KURONO_RS14105 2984090..2985217 - 1128 WP_003899420.1 site-specific integrase -
KURONO_RS14110 2985362..2985589 - 228 WP_003899421.1 hypothetical protein -
KURONO_RS21640 2985586..2985975 - 390 WP_003899422.1 hypothetical protein -
KURONO_RS14115 2985881..2986153 + 273 WP_003900544.1 hypothetical protein -
KURONO_RS14120 2986252..2986485 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2974950..2985217 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-105)

Antitoxin

Antitoxin MazE-like

(13-72)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T30746 WP_003899414.1 NZ_AP014573:c2981308-2980985 [Mycobacterium tuberculosis str. Kurono]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T30746 NZ_AP014573:c2981308-2980985 [Mycobacterium tuberculosis str. Kurono]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT30746 WP_003899415.1 NZ_AP014573:c2981633-2981388 [Mycobacterium tuberculosis str. Kurono]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT30746 NZ_AP014573:c2981633-2981388 [Mycobacterium tuberculosis str. Kurono]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References